Liquid-fermented high-yield polysaccharide bacterial strain of coriolus versicolor and breeding method thereof
A liquid fermentation, mycelium polysaccharide technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problem of few reports on the breeding of new varieties of Versicolor versicolor, and improve the efficiency of mutagenesis and the probability of positive mutation, the effect of increasing the yield and not easy to degenerate
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[0026] The technological method for mutagenic screening of new strains of polysaccharides produced by liquid fermentation of Versicolor versicolor, the steps of which are as follows:
[0027] 1. Screening of high-yielding polysaccharides from Yunzhi mycelia
[0028] Different varieties of versicolor versicolor strains SYZ1, SYZ2, SYZ3, SYZ4, SYZ5, SYZ6, SYZ7 and SYZ8 were introduced from China. The strains come from the Chinese Academy of Agricultural Sciences, Northeast China, and the strains preserved in this research institute. They were inoculated on PDA slant medium, and cultured at 28°C in the dark for 7 days for activation. The activated bacterial strain was inoculated in the center of the petri dish equipped with PDA medium, the time of versicolor versicolor mycelium germination was recorded, the growth rate was measured, and the morphology of the mycelia (thickness and denseness of the mycelia) was observed. Then carry out the Yunzhi liquid fermentation test, quantit...
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