Liquid-fermented high-yield polysaccharide bacterial strain of coriolus versicolor and breeding method thereof

A liquid fermentation, mycelium polysaccharide technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problem of few reports on the breeding of new varieties of Versicolor versicolor, and improve the efficiency of mutagenesis and the probability of positive mutation, the effect of increasing the yield and not easy to degenerate

Active Publication Date: 2016-01-06
JIANGSU ANHUI BIO TECH +1
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Problems solved by technology

[0006] At present, there are not many reports on the breeding of new varieties of Versicolor versicolor, and there are no relevant reports at home and abroad about the use of ion

Method used

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  • Liquid-fermented high-yield polysaccharide bacterial strain of coriolus versicolor and breeding method thereof
  • Liquid-fermented high-yield polysaccharide bacterial strain of coriolus versicolor and breeding method thereof
  • Liquid-fermented high-yield polysaccharide bacterial strain of coriolus versicolor and breeding method thereof

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Experimental program
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Embodiment Construction

[0026] The technological method for mutagenic screening of new strains of polysaccharides produced by liquid fermentation of Versicolor versicolor, the steps of which are as follows:

[0027] 1. Screening of high-yielding polysaccharides from Yunzhi mycelia

[0028] Different varieties of versicolor versicolor strains SYZ1, SYZ2, SYZ3, SYZ4, SYZ5, SYZ6, SYZ7 and SYZ8 were introduced from China. The strains come from the Chinese Academy of Agricultural Sciences, Northeast China, and the strains preserved in this research institute. They were inoculated on PDA slant medium, and cultured at 28°C in the dark for 7 days for activation. The activated bacterial strain was inoculated in the center of the petri dish equipped with PDA medium, the time of versicolor versicolor mycelium germination was recorded, the growth rate was measured, and the morphology of the mycelia (thickness and denseness of the mycelia) was observed. Then carry out the Yunzhi liquid fermentation test, quantit...

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Abstract

The invention discloses a liquid-fermented high-yield polysaccharide bacterial strain of coriolus versicolor and a breeding method thereof. The preservation number of the bacterial strain is CGMCC No. 10903; the classification name of the bacterial strain is coriolus versicolor in Latin. The polysaccharide yield of the coriolus versicolor mycelium mutated through the method is higher than that of a coriolus versicolor mutant strain of an original strain, the yield of coriolus versicolor polysaccharide is improved, and high economic benefits can be created.

Description

technical field [0001] The invention relates to a high-yielding polysaccharide strain of Yunzhi liquid fermentation and a breeding method thereof. Background technique [0002] Coriolus versicolor intracellular polysaccharides (Coriolusversicolorintracellu—larpolysaccharides) is a binding proteoglycan extracted and purified from the fungus of the genus Yunzhi of the basidiomycetes Polyporaceae Coriolus lucidum or the cultured mycelia. It has various pharmacological effects such as immunity, reducing radiation and chemotherapy side effects, and has anti-HIV activity. Due to the limited resources of Versicolor versicolor in the wild, the use of solid fermentation to cultivate mycelium to extract polysaccharides has high cost, long cycle, unstable product quality, and low yield. Moreover, my country's Drug Administration recently required that the culture of Yunzhi mycelia should be changed from solid culture to liquid culture. The body extracts intracellular polysaccharides, s...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N15/01C12R1/645
Inventor 陈惠郑惠华刘广建全卫丰薛璟蒋益季宏更汪洁汪少芸田贞乐吴伟杰
Owner JIANGSU ANHUI BIO TECH
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