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snp markers and their applications

A marker and goat technology, applied in the direction of recombinant DNA technology, DNA/RNA fragments, microbial determination/inspection, etc.

Active Publication Date: 2018-06-08
深圳华大基因农业控股有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the genetic markers related to wool curl found in the research on the genetic mechanism of goat wool curl are preliminary results, and are limited to the existing candidate genes. Using these results, it is difficult to make more accurate selection for the trait of goat wool curl

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Acquisition of SNP markers associated with goat wool curl

[0039] The inventors used RAD-seq and genome-wide association analysis (GWAS) to mine green goat wool curl-related genetic markers, and using these markers can improve the accuracy of wool curl trait selection. Specific steps are as follows:

[0040] 1. Collection of blood samples and DNA extraction of green goats

[0041] 1) 457 blood samples were collected from green goats of Shandong Yongwang Animal Husbandry Development Co., Ltd., and the statistics of wool curl characteristics are shown in Table 1.

[0042] 2) Genomic DNA was extracted by the phenol-chloroform method.

[0043] Table 1 Statistical results of curly properties of green goat wool

[0044] character

straight hair

curly hair

total

number of individuals

431

26

457

[0045] 2. RAD-seq library construction

[0046] Library construction method reference (Rapid SNP Discovery and Genetic Mapping...

Embodiment 2

[0064] Example 2 Sequencing verification of SNP markers associated with goat wool curls

[0065] 2.1 Extract the genomic DNA in the blood sample of the green goat to be tested

[0066] The blood samples of the green goats to be tested were from the green goats of Shandong Hanlong Goat Industry Co., Ltd., 5 samples were randomly selected, and the genomic DNA was extracted according to the DNA extraction method described in Example 1.

[0067] 2.2 Amplification of nucleotide fragments containing SNP sites

[0068] Using the genomic DNA in the blood samples of the green goats to be tested obtained by the aforementioned extraction as a template, using the forward primer F: CAAGGTTTAACAAGGCAGTT (SEQ ID NO: 2) and the reverse primer R: CTGTTGATCCAAGTGAACTT (SEQ ID NO: 3), amplified The nucleotide fragment where the SNP to be tested is located. The amplification system of this PCR amplification is calculated as 20 μl: 2 μl of template DNA of 25-50 ng / μl, 0.3 μl of each primer shown...

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Abstract

The invention discloses an SNP marker and the application thereof, wherein the SNP marker is the basic group C or T in the position 5119569 of the chromosome No. 26 of a goat. The SNP marker provided by the invention is in close relation to wool crimp characters of goats, and can be effectively used for molecular marker assistant breeding of goats.

Description

technical field [0001] The present invention relates to SNP markers and their applications. Specifically, the present invention relates to SNP markers related to the curly traits of goat wool, primer pairs and kits for detecting the aforementioned SNP markers, the use of the aforementioned SNP markers, primer pairs, and kits in goat breeding, and detection of goat wool curl trait method. Background technique [0002] China is the country with the most abundant goat breed resources in the world. Goats can be divided into cashmere, wool, fur, lamb skin, meat and milk goats according to their different uses. Goat hair is generally divided into inner and outer layers. The inner layer is the unmyelinated hair fiber formed by the secondary hair follicles in the skin. Cashmere is thin and soft, has good luster and strong thermal insulation performance. It is known as "soft gold" and is usually used as an important textile material for export in my country. The outer coarse hair ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/6888
Inventor 张清峰张耕耘夏秋菊宋峰峰苗泽圃范艺翔陈庆春王宇
Owner 深圳华大基因农业控股有限公司