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Measurement method of content of anthocyanin in leaves of peony on the basis of hyperspectrum

A technology of anthocyanin content and peony leaves, which is applied in the direction of color/spectral characteristic measurement and climate change adaptation, can solve the problems of low anthocyanin content, time-consuming and laborious measurement, and large differences in anthocyanin content of peony leaves. Achieve the effect of simple method, high accuracy and high precision of prediction model

Inactive Publication Date: 2016-01-13
HENAN UNIV OF SCI & TECH
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Problems solved by technology

For peony plants, during the flowering stage, the anthocyanin content of the leaves of different varieties of peony varies greatly, and the anthocyanin content is relatively low, and laboratory measurement is time-consuming and laborious.
At present, the determination of anthocyanins in peony leaves based on hyperspectral monitoring models has not been reported

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  • Measurement method of content of anthocyanin in leaves of peony on the basis of hyperspectrum
  • Measurement method of content of anthocyanin in leaves of peony on the basis of hyperspectrum
  • Measurement method of content of anthocyanin in leaves of peony on the basis of hyperspectrum

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Embodiment 1

[0026] In the present embodiment, the method for determining the content of anthocyanins in leaves of peony based on hyperspectrum comprises the following steps:

[0027] 1. Establish a spectroscopic monitoring model for anthocyanin content in peony leaves (see flow chart figure 1 )

[0028] 1) Sampling and hyperspectral data acquisition

[0029] At the beginning of April of a certain year, in a peony garden of a certain university, 14 peony leaves of different varieties were collected in the early flowering stage of peony (Paeonia Suffruticosa). The value is 350-2500nm, the spectral resolution of the 350-1000nm band is ≤3.5nm, the spectral resolution of the 1000-1850nm band is ≤9.5nm, and the spectral resolution of the 1850-2500nm band is ≤6.5nm; The light source is a built-in tungsten halogen lamp; before each measurement, the diffuse reflectance reference plate is used to optimize the instrument) reflectance spectrum data, each leaf is measured at 3 positions, each positi...

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Abstract

The invention discloses a measurement method of content of anthocyanin in leaves of peony on the basis of hyperspectrum and belongs to the technical field of lossless monitoring of crop growth information in precision agriculture. The measurement method includes the following steps: fusing reflection spectrum data of leaves of peony with anthocyanin content data; determining a sensitive waveband of the anthocyanin content through correlation analysis; establishing an anthocyanin content spectrum monitoring model based on characteristic wavebands in selected software; measuring the reflection spectrum data in the characteristic wavebands of the peony leaves through a hyperspectrum radiometer; introducing the data into the spectrum monitoring model; and calculating the content of the anthocyanin in the peony leaves. The measurement method, compared with a wet chemical method in the prior art, has following advantages: 1) the method is free of sampling due to on-site measurement, so that the peony leaves are free of damage; 2) the method allows repeated measurement, thereby achieving long-time monitoring; and 3) all effective waveband information being sensitive to the content of anthocyanin are employed, so that the prediction model is high in accuracy and is especially suitable for quickly and accurately estimating the peony leaves being medium or low in content of the anthocyanin.

Description

technical field [0001] The invention relates to a hyperspectral-based method for measuring anthocyanin content in peony leaves, which belongs to the technical field of non-destructive monitoring of crop growth information in precision agriculture. Background technique [0002] The change of pigment content in plant leaves can not only reflect the growth and nutritional status of plants, but also reflect the response of plants to environmental factors. Therefore, leaf pigment is one of the most commonly measured parameters in plant research. Anthocyanins are water-soluble flavonoids, which are the third major pigment in plant leaf pigments, and are abundant in young and old leaves of plants. Anthocyanins can restore the light environment of leaves, potentially regulate the ability of photosynthesis to limit photoinhibition and photobleaching, and the ability to defend against photodamage. Anthocyanins can be used as osmotic adjustment substances to improve the ability of pla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25
CPCY02A40/10
Inventor 刘秀英熊建利常庆瑞严林王力宋荣杰马文君
Owner HENAN UNIV OF SCI & TECH
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