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Preparation method for Armillaria luteo-virens sporophore sterol compound with anti-liver cancer activity

A technology of Armillaria yellow-green fungus and Armillaria yellow-green is applied in the directions of biochemical equipment and methods, determination/inspection of microorganisms, etc., and can solve the problems such as lack of research on the chemical composition of the fruiting body of Armillaria yellow-green, and achieve quality Stable and controllable, easy to judge, simple process effect

Inactive Publication Date: 2016-01-20
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on the chemical constituents of the fruiting bodies of Armillaria chrysanthemum is also extremely scarce in the literature

Method used

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  • Preparation method for Armillaria luteo-virens sporophore sterol compound with anti-liver cancer activity
  • Preparation method for Armillaria luteo-virens sporophore sterol compound with anti-liver cancer activity
  • Preparation method for Armillaria luteo-virens sporophore sterol compound with anti-liver cancer activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 A preparation method of Armillaria chrysanthemum fruiting body anti-liver cancer active sterol compounds, comprising the following steps:

[0042] (1) Acetone extract of Armillaria chrysanthemum was added to 1 times its volume of 50% n-hexane-ethyl acetate mixture for dissolving, and passed through a 0.45 μm organic filter membrane after dissolving to obtain a sample solution containing acetone extract; The sample solution of the acetone extract was separated on the preparative chromatography with 10 μm silica gel as the separation matrix and dried under reduced pressure to obtain Fr1, Fr2, Fr3, Fr4, Fr5, Fr6, Fr7, Fr8, Fr9, Fr10, Fr12, , Fr13, A total of 14 standardized component samples of Fr14;

[0043] in:

[0044] The conditions for concentration under reduced pressure refer to a vacuum degree of 0.06 MPa and a temperature of 70°C.

[0045] The conditions for preparation of chromatographic separation refer to the size of the chromatographic column is...

Embodiment 2

[0065] Example 2 A preparation method of a sterol compound with anti-liver cancer activity in the fruiting bodies of Armillaria chrysanthemum, comprising the following steps:

[0066] (1) Acetone extract of Armillaria chrysanthemum was added into 6 times its volume of 10% n-hexane-ethyl acetate mixture for dissolving, and passed through a 0.45 μm organic filter membrane after dissolving to obtain a sample solution containing acetone extract; The sample solution of the acetone extract was separated on a preparative chromatography using 10 μm silica gel as the separation matrix and dried under reduced pressure to obtain a total of 14 standardized component samples of Fr1, Fr2, ..., Fr13, and Fr14;

[0067] in:

[0068] The conditions for concentration under reduced pressure refer to a vacuum degree of 0.09 MPa and a temperature of 50°C.

[0069] The conditions for preparative chromatographic separation are the same as in Example 1 step (1).

[0070] (2) Use the iCelligence rea...

Embodiment 3

[0076] Example 3 A preparation method of a sterol compound with anti-liver cancer activity in the fruiting bodies of Armillaria chrysanthemum, comprising the following steps:

[0077] (1) Acetone extract of Armillaria chrysanthemum was added into 3 times its volume of 30% n-hexane-ethyl acetate mixture for dissolving, and after dissolving, passed through a 0.45 μm organic filter membrane to obtain a sample solution containing acetone extract; The sample solution of the acetone extract was separated on a preparative chromatography using 10 μm silica gel as the separation matrix and dried under reduced pressure to obtain a total of 14 standardized component samples of Fr1, Fr2, ..., Fr13, and Fr14;

[0078] in:

[0079] The conditions for concentration under reduced pressure refer to a vacuum degree of 0.08 MPa and a temperature of 60°C.

[0080] The conditions for preparative chromatographic separation are the same as in Example 1 step (1).

[0081] (2) Use the iCelligence real...

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Abstract

The invention discloses a preparation method for an Armillaria luteo-virens sporophore sterol compound with anti-liver cancer activity. The preparation method has the advantages of simple process, a high recovery rate, good repeatability and stable and controllable quality. Moreover, a cell growth curve is used as a basic index in the invention to determine influence of standardized components and samples on cell physiological activities like adherence and breeding, and obtained results are visual and easy to determine. According to analysis results of NMR hydrogen spectrum and carbon spectrum, obtained Armillaria luteo-virens sporophore anti-liver cancer sterol active samples (including a sample No. Fr8-1 and a sample No. Fr8-4) are determined to be 3beta-hydroxy-(22E,24R)-ergosta-5,8,22-trien-7-one and (3alpha,5alpha),(8beta,11beta)-diepidioxy-ergost-22E-en-12-one, respecitvely.

Description

technical field [0001] The invention relates to the technical fields of medicinal chemistry and biomedicine, in particular to a preparation method and an application of a sterol compound with anti-liver cancer activity of Armillaria chrysanthemum fruiting bodies. Background technique [0002] Hepatocellular carcinoma (hepatocellular carcinoma, HCC, hereinafter referred to as liver cancer) is a highly malignant tumor with a dangerous prognosis. The 5-year survival rate is only about 7%, and it is the fifth most common malignant tumor in the world. The highest rate ranks third. The incidence of liver cancer in my country accounts for more than half of the world, and the age of onset tends to be younger. At present, the annual mortality rate of liver cancer in my country is about 20 / 100,000, ranking second in the mortality rate of various malignant tumors in my country, and has been on the rise in the past 10 years. Liver cancer has become a major killer that seriously threat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
Inventor 党军王启兰陶燕铎邵赟梅丽娟张莉崔玉磊
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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