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SNP (single nucleotide polymorphism) marker relevant to prevention of colorectal cancer by aid of non-steroidal anti-inflammatory medicines and application of SNP marker

A colorectal cancer, non-steroidal technology, applied in the field of SNP markers, can solve the problems of colorectal cancer preventive drug guidance and auxiliary diagnosis without SNP application

Active Publication Date: 2016-01-27
NANJING YIKE POPULATION HEALTH RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the application of NSAID-related SNPs in the guidance of preventive medication and auxiliary diagnosis of colorectal cancer.

Method used

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  • SNP (single nucleotide polymorphism) marker relevant to prevention of colorectal cancer by aid of non-steroidal anti-inflammatory medicines and application of SNP marker
  • SNP (single nucleotide polymorphism) marker relevant to prevention of colorectal cancer by aid of non-steroidal anti-inflammatory medicines and application of SNP marker
  • SNP (single nucleotide polymorphism) marker relevant to prevention of colorectal cancer by aid of non-steroidal anti-inflammatory medicines and application of SNP marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] The collection of embodiment 1 sample and the arrangement of sample data

[0084] The inventor has selected 1202 samples that meet the following criteria to carry out the experimental samples of a single SNPSequenomMassARRAY genotyping:

[0085] 1. 568 patients with colorectal cancer diagnosed by pathology;

[0086] 2. 634 healthy controls matched with the age and sex of the cases;

[0087] The demographic data and clinical data of these samples were systematically collected, and whether the individual cases were regularly taking non-steroidal anti-inflammatory drugs was extracted through the clinical course records. There were 362 patients who regularly took non-steroidal anti-inflammatory drugs. , 206 patients did not take non-steroidal anti-inflammatory drugs. The range of drugs includes acetylsalicylate (such as aspirin), non-salicylate and non-acetylsalicylate drugs (such as Fenbid, ibuprofen, nimesulide, Voltaren, etc.).

Embodiment 2

[0088] Example 2 Genotyping and scanning of SNP markers in peripheral blood DNA

[0089] Among the 568 colorectal cancer patients (including 362 in the non-steroidal anti-inflammatory drug group and 206 in the non-steroidal anti-inflammatory drug group) and 634 normal controls, the case-control age and gender were matched. The two groups of people were detected by SequenomMassARRAY typing to obtain relevant results. The specific steps are:

[0090] 1. Add hemolysis reagent (i.e., lysate, 40 parts) to the peripheral blood stored in a 2ml cryopreservation tube. The volume of the solution was adjusted to 2000ml, the same below), and it was completely transferred after inverting and mixing.

[0091] 2. Removal of red blood cells: Fill the 5ml centrifuge tube to 4ml with hemolysis reagent, mix by inverting, centrifuge at 4000rpm for 10 minutes, and discard the supernatant. Add 4ml of hemolysis reagent to the precipitate, invert and wash again, centrifuge at 4000rpm for 10 minute...

Embodiment 3

[0110] Example 3 Using the risk scoring method to further analyze the relationship between SNP and colorectal cancer

[0111] According to the above results, the present inventors stratified the two groups of samples on whether to take non-steroidal anti-inflammatory drugs (i.e. "taking non-steroidal anti-inflammatory drug colorectal cancer group" and "not taking non-steroidal anti-inflammatory drug Colorectal cancer group"), the genotype distribution frequency was compared, and finally 15 positively associated SNPs were selected, and the single SNP regression coefficient was used as the weight to further obtain the risk score, and the ROC was drawn to evaluate the diagnostic sensitivity and Specificity, and then analyze these SNPs to diagnose whether it is suitable to take non-steroidal anti-inflammatory drugs to prevent colorectal cancer. A combined analysis of 15 SNP markers found that these 15 SNPs separated colorectal cancer patients who were NSAID-eligible from those who...

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Abstract

The invention belongs to the field of genetic engineering and tumor medicine, and discloses an SNP (single nucleotide polymorphism) marker relevant to prevention of colorectal cancer by the aid of non-steroidal anti-inflammatory medicines and application of the SNP marker. The SNP marker is a composition of rs12217975, rs1927466, rs7586006, rs17868336, rs1500482, rs16973225, rs10505806, rs6717546, rs2965667, rs73172231, rs16825767, rs1902023, rs2745559, rs73172243 and rs2920421. The SNP marker and the application have the advantages that the SNP marker can be used for guiding prevention and treatment clinical medication of the non-steroidal anti-inflammatory medicines for colorectal patients, accordingly, medicine abuse can be reduced, and excessive medical treatment can be prevented.

Description

field of invention [0001] The invention belongs to the fields of genetic engineering and tumor medicine, and relates to SNP markers related to the prevention of colorectal cancer by non-steroidal anti-inflammatory drugs and applications thereof. Background technique [0002] Colorectal cancer is one of the most common digestive tract tumors in the world. It is the third most common tumor in men and the second in women, and ranks second in cancer deaths. According to the International Agency for Research on Cancer of the World Health Organization, there were about 1.2 million new cases of colorectal cancer in the world in 2008, and its mortality rate accounted for about 8% of all malignant tumors. In my country, with the development of society and economy and the continuous changes in residents' lifestyle and eating habits, the incidence and mortality of colorectal cancer are showing an increasing trend year by year, ranking third in the incidence of malignant tumors and fift...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/106C12Q2600/156
Inventor 王一雄江玥戴俊程李绵丁舒丹
Owner NANJING YIKE POPULATION HEALTH RES INST CO LTD
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