Construction of algal toxin Raman sensor adopting gold nanoflower-silver nanoparticle bimetallic nano assembly

A technology of silver nanoparticles and bimetallic nanometers, applied in Raman scattering, material excitation analysis, etc., can solve the problems that the detection of algal toxins has not yet been established, and achieve the rapid effect of algal toxins

Active Publication Date: 2016-01-27
JIANGNAN UNIV
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Problems solved by technology

However, the detection of algal toxins using Raman aptasensors has not been established so far.

Method used

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  • Construction of algal toxin Raman sensor adopting gold nanoflower-silver nanoparticle bimetallic nano assembly
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  • Construction of algal toxin Raman sensor adopting gold nanoflower-silver nanoparticle bimetallic nano assembly

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Embodiment 1

[0023] (1) Synthesis of gold nanoflowers

[0024] Add 1.25mL of 5mM chloroauric acid solution into 23.75mL of ultrapure water, mix well, heat rapidly to boiling, and vigorously stir for 5min under boiling conditions. Quickly add 500 μL of freshly prepared sodium citrate with a mass fraction of 1%, and react for 10 minutes. Under vigorous stirring, 500 μL of the above solution was quickly added to the mixed system of 500 μL of 1% polyvinylpyrrolidone and 1 mL of 0.1M phosphate buffer solution, and 300 μL of 10 mM chloroauric acid solution and 300 μL of 10 mM hydroxylamine hydrochloride solution were added. After reacting for 2 h, the mixture was centrifuged at 5000 rpm for 5 min, and the precipitate was dissolved in 100 μL of ultrapure water.

[0025] (2) Surface modification of gold nanoflowers and silver nanoparticles

[0026] Take 100 μL of the gold nanoflower solution prepared above, add 10 μL of 10 μM thiol-modified probe 1, and the molar ratio of gold nanoflower and pro...

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Abstract

The invention relates to construction of an algal toxin Raman sensor adopting a gold nanoflower-silver nanoparticle bimetallic nano assembly and belongs to the field of material chemical application. The construction mainly comprises the contents that a simple and convenient synthetic method of gold nanoflowers with controllable surface bulges is provided; by controlling concentration of hydroxylamine hydrochloride, gold nanoflower structures with different bulge sizes can be obtained; based on a Y-shaped aptamer assembling configuration, the old nanoflower-silver nanoparticle bimetallic nano assembly is prepared; a spacing size and intensity variation of an electromagnetic field of the nano assembly under the condition of multiple hot spots between double metals are researched; Raman signal amplifying intensity of the assembly is analyzed; and the Raman sensor with a high speed, high specificity and high sensitivity for algal toxin is constructed.

Description

technical field [0001] The invention establishes a algae toxin Raman sensor of a gold nanoflower-silver nanoparticle bimetallic nanoassembly, which belongs to the field of material chemistry application. Background technique [0002] Microcystin is the most toxic substance produced by cyanobacteria under the conditions of overnutrition, strong light, and high water temperature. Among them, cyanotoxin-LR is the most common toxin among cyanotoxins. The World Health Organization has proposed that the maximum allowable concentration of cyanotoxin-LR in drinking water is 1nM. Currently, electrochemical biosensors, high performance liquid chromatography and immunoassays are mainly used to detect algal toxin-LR with high sensitivity. However, the application of electrochemical biosensors is limited due to repeated polishing of electrodes and relatively cumbersome steps; high-performance liquid chromatography is time-consuming and has low sensitivity; immunoassays depend on the sta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 赵媛罗耀东宋启军杨璇
Owner JIANGNAN UNIV
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