Novel malaria whole worm inactivated vaccine with preventing and treating effects
A technology for vaccines and malaria, which is applied in the field of new-type inactivated malaria whole-worm vaccines, can solve the problems of large dosage, and achieve the effects of simple preparation, immune response regulation and enhanced effect
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Embodiment 1
[0025] Preparation of embodiment 1 inactivated whole worm vaccine
[0026] Firstly, the parasite antigen is prepared, and the preparation process is as follows:
[0027] Resuscitate the Plasmodium NSM strains stored in liquid nitrogen, then infect 30 Balb / c mice with the resuscitated NSM, and collect NSM parasites as parasites when the parasitemia reaches about 50% on the 4th day and the 9th day after infection, respectively. body antigen. The main process of the parasite antigen acquisition process is as follows: the blood components of the mouse are obtained by taking blood from the heart, and added to a centrifuge tube containing heparin to avoid coagulation; the above blood cells are washed 3 times with PBS with a pH value of 7.4 and a concentration of 0.1mol / L After heparin was removed, saponin with a final concentration of 0.5% was added to lyse red blood cells to release intracellular parasite antigens; the above components were washed 5 times with PBS to remove heme a...
Embodiment 2
[0029] Example 2 Novel inactivated whole worm vaccine enhances the protection against red inner stage of malaria
[0030] The inactivated whole worm vaccine prepared in Example 1 was used to immunize mice with 1×10 8 The mice were subcutaneously immunized with a dose of Plasmodium per mouse, and the immunization was repeated twice every other week. The untreated mice were used as the blank control; after the last immunization, 1 × 10 6 Each challenged the mice of the blank control group and the experimental group, took blood smears for 48 hours to observe the immune protection effect of the mice, and counted and analyzed the protozoaemia of the mice. Statistical results such as figure 1 As shown, the blank control was subjected to 1×10 6 After a erythrocytic Plasmodium challenged, its parasitemia was at a high level all the time, and when the parasitemia was 30-40%, the mice all died, while its parasitemia of the inactivated whole worm vaccine group in Example 1 was at a hig...
Embodiment 3
[0031] Example 3 Novel inactivated whole worm vaccine enhances the protection to the infrared stage of malaria
[0032] The method described in Example 2 was used to immunize mice with the inactivated whole worm vaccine. The mice in the control group were not immunized. After the last immunization, the mice in the control group and the experimental group were challenged with 5000 Plasmodium parasites in the infrared stage, and the liver was taken after 42 hours. Quantitative detection of insect load, the results are as follows figure 2 As shown, compared with the control group, the hepatic stage worm load in the inactivated whole worm vaccine group decreased by about 50%. Immunity effect (statistically significant).
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