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Polypeptide used for preventing and treating enteropathogenic Escherichia coli infection

A technology of Escherichia coli and Escherichia coli, which is applied in the direction of gene therapy, peptides, antibacterial drugs, etc., can solve the problems of difficult infection treatment and easy drug resistance, and achieve the effect of reducing adsorption

Active Publication Date: 2016-02-03
SHENZHEN NANSHAN DISTRICT PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of antibiotics for treatment is prone to drug resistance, causing drug-resistant mutant bacteria to accumulate in the human body, making it difficult to treat future infections

Method used

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  • Polypeptide used for preventing and treating enteropathogenic Escherichia coli infection
  • Polypeptide used for preventing and treating enteropathogenic Escherichia coli infection
  • Polypeptide used for preventing and treating enteropathogenic Escherichia coli infection

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Embodiment Construction

[0017] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0018] 1. In vitro expression of EspB protein;

[0019] The EspB fragment was amplified from the E2348 / 69 (O127:H6) strain, and the gene fragment was inserted into the BamHI and EcoRI sites of the pET21b expression vector using standard molecular methods to construct a pET21b containing 6 (His) histidine residues- EspB expression vector. The recombinant Escherichia coli transfected with pET21b-EspB expression vector was induced to express EspB protein by adding IPTG to the medium. After adding 1 mM IPTG, the cells were collected by centrifugation, and the cells were suspended using Qiagen Company A buffer, which was 20 mM Tris produced by A buffer, 500 mM NaCl, pH9. After the cells were lysed by sonication, the cell suspension was centrifuged, and the supernatant was collected for Ni 2+ - Exchange membrane (Qiagen) filtration. Th...

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Abstract

The present invention provides a polypeptide for preventing and treating enteropathogenic Escherichia coli infection. The polypeptide comprises an amino acid sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3 or SEQ ID NO: 4. According to the technical scheme of the present invention, the affinity of the polypeptide for preventing and treating enteropathogenic Escherichia coli infection and EspB protein is higher than that of control protein, the polypeptide for preventing and treating enteropathogenic Escherichia coli infection can inhibit adhesion of EPEC an HEp 2 cells and interrupt functions of EspB, thereby reducing epithelial cell adsorption by the EPEC, providing a new direction for EPEC treatment and laying a foundation for researching and developing drugs for preventing and treating the EPEC.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a polypeptide for preventing and treating enteropathogenic Escherichia coli infection. Background technique [0002] Currently, broad-spectrum antibiotics are used in the treatment of enteropathogenic Escherichia coli, and there is no specific inhibitor against this type of bacteria. The use of antibiotics for treatment is prone to drug resistance, and the drug-resistant mutant bacteria will gather in the human body, which will bring difficulties to the treatment of future infections. [0003] Enteropathogenic E. coli (EPEC) is one of the important pathogenic bacteria that cause diarrhea in infants and young children and sporadic diarrhea in adults around the world. A / E injury) leads to intestinal mucosal injury. The characteristic of enteropathogenic Escherichia coli EPEC infection is that the pathogenic bacteria can instinctively adhere to the host cell membrane, dest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C12N15/11A61K38/10A61K48/00A61P31/04
CPCY02A50/30
Inventor 邓启文余治健陈重程航邓向斌李多云郑金鑫
Owner SHENZHEN NANSHAN DISTRICT PEOPLES HOSPITAL
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