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A kind of clam clam active hexapeptide and its preparation method and application

A clam clam active technology, applied in the field of clam clam active hexapeptide and its preparation, can solve the problems of different flavors and shapes, and achieve the effect of mild enzymatic hydrolysis, low cost, and inhibition of cell proliferation activity

Active Publication Date: 2021-01-29
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The shape of razor clam and razor clam is different, and the flavor is slightly different when eating. At present, the research on enzymatic polypeptide of razor clam has not been reported. It is of great significance to increase the economic value of clam clams with multi-basic functional foods and medicines

Method used

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  • A kind of clam clam active hexapeptide and its preparation method and application
  • A kind of clam clam active hexapeptide and its preparation method and application
  • A kind of clam clam active hexapeptide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the preparation of clam clam active hexapeptide

[0031] 1. Pre-processing:

[0032] Take fresh razor clams, remove the shells and take the razor clams meat, wash them and drain them for later use.

[0033] 2. Alkaline proteolysis

[0034] 2.1 Use a high-speed tissue masher to mash the razor clam meat, add distilled water to homogenate, the ratio of material to liquid is 1:1~3, accurately weigh the homogenate, and use 0.1mol / L hydrochloric acid solution and 0.1mol / L NaOH solution Adjust the pH value of the homogenate, add alkaline protease for enzymolysis for several hours, the enzymolysis conditions are: enzymolysis temperature 35-50°C, pH 8-10.4, enzymolysis time 2-8h, enzyme amount 300-1200U / g razor clam Meat. After the enzymatic hydrolysis, inactivate the enzyme at 100°C for 15 minutes, centrifuge at 4°C for 15 minutes (10000r / min), and take the supernatant for later use.

[0035] 2.2 Alkaline proteolysis process optimization

[0036] 2.2.1 Alkali...

Embodiment 2

[0061] Example 2: Study on the Anti-PC-3 Cell Proliferation Activity of Razor Razor Active Hexapeptide

[0062] Taking the F12 medium as the control group, the above-mentioned MTT method was used to compare the effects of different concentrations and different action times of the active hexapeptide of razor clam on the proliferation of PC-3 cells. The test results are as follows: Figure 4 As shown, it can be seen that the active hexapeptide of razor clam can effectively inhibit the proliferation of PC-3 in a concentration- and time-dependent manner.

[0063] Main materials and reagents used in the above-mentioned each embodiment:

[0064] Razor clams were purchased from Nanzhen Market, Zhoushan City, Zhejiang Province; prostate cancer cell PC-3 hormone-independent cells (originally purchased from Shanghai Cell Bank, Chinese Academy of Sciences, and preserved by our laboratory); fetal bovine serum (FBS), Hangzhou Sijiqing Biology Products Co., Ltd.; HamF12 medium, Gibco Compa...

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Abstract

The invention relates to an active hexapeptide of bamboo razor, the amino acid sequence of which is: Val-Pro-Trp-Asp-Thr-Asp, and the molecular weight detected by ESI / MS is 731.7 Da; it is prepared by the following steps: fresh bamboo razor is peeled and meat is obtained , the bamboo clams meat is washed and then smashed, and the tissue is homogenized, and the pH value of the homogenate is adjusted for subsequent use; alkaline protease is added to the above-mentioned homogenate to carry out enzymolysis, and the enzymolysis sample is obtained, and the obtained enzymolysis sample is subjected to ultrafiltration. Filtration, G-25 gel separation reversed-phase high-performance liquid chromatography purification, collection of elution peak components and amino acid sequencing to obtain the bamboo clams active hexapeptide. The maximum proliferation inhibition rate of the bamboo razor active hexapeptide on human prostate cancer PC-3 cells can reach more than 90%, which can provide a theoretical basis for the research of anti-prostate cancer drugs. The bamboo razor active hexapeptide is obtained by biological enzymatic hydrolysis. The source of raw materials is wide and the cost is low, which is of great significance for further research and development of medicines and foods based on bamboo clams, and for improving the economic value of bamboo clams.

Description

technical field [0001] The invention relates to the field of bioactive peptides, in particular to a clam clam active hexapeptide and its preparation method and application. Background technique [0002] Razor clam, also known as the king of razor clam, is a marine bivalve mollusc with an elongated body and a bamboo tube shape after the two shells are closed, so it has the name of razor clam. Razor clam shells are smooth, yellowish-brown, shiny, brittle and thin, with a prominent surface, the back edge is parallel to the belly edge, the rear end is purely round, rectangular, with obvious growth lines, about 11 cm long. It is distributed along the north and south coasts of my country. The meat is delicious and can be eaten fresh or dried. Bamboo razor clam meat tastes sweet and salty, cold in nature, and enters the heart, liver, and kidney meridian; it has the effects of nourishing yin, clearing away heat, eliminating annoyance, and detoxifying alcohol; It has a certain ther...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K1/36C07K1/34C07K1/20C07K1/16C12P21/06A61K38/08A61P35/00A23L33/18
Inventor 黄芳芳丁国芳杨最素余方苗
Owner ZHEJIANG OCEAN UNIV