Proline concentration detection method based on biosensing technology

A biosensing and concentration detection technology, applied in the direction of electrochemical variables of materials, can solve problems such as damage or damage to sample materials, and achieve the effect of simple operation and fast detection speed.

Active Publication Date: 2016-02-10
BEIJING RES CENT OF INTELLIGENT EQUIP FOR AGRI
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Problems solved by technology

Moreover, the existing proline detection technology uses in vitro sampling, which will cause damage or damage to the sample material, especially precious materials may cause complete damage

Method used

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  • Proline concentration detection method based on biosensing technology
  • Proline concentration detection method based on biosensing technology

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Embodiment Construction

[0010] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are the Some, but not all, embodiments are invented. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0011] Such as figure 1 As shown, the present embodiment discloses a proline concentration detection method based on biosensing technology, including:

[0012] S1, integrating the counter electrode, reference electrode and working electrode into a microelectrode array (the microelectrode array is prepared by etching technology, spraying technology, electrodeposition te...

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Abstract

The invention discloses a proline concentration detection method based on the biosensing technology. The method can achieve accurate detection of the proline concentration in a to-be-detected organ under the condition that the to-be-detected organ of a to-be-detected plant is not damaged. The method comprises the following steps: S1, integrating an electrode, a reference electrode and a working electrode into a microelectrode array; S2, putting the detection end of the electrode on the microelectrode array into the to-be-detected part of the to-be-detected plant, collecting the anodic peak current generated through the electrochemical reaction of proline in the to-be-detected part through using an electrochemical workstation and adopting the cyclic voltammetry, calculating the average value of the current in preset duration after the current change becomes steady, and substituting the average value into a precomputed linear formula of the proline concentration and the average value of the current, so as to obtain the proline concentration of the to-be-detected part.

Description

technical field [0001] The invention relates to the field of plant proline detection, in particular to a proline concentration detection method based on biosensing technology. Background technique [0002] The synthesis, metabolism, transport and accumulation of bioactive small organic molecules in plants run through the whole growth cycle of plants and affect the life processes of plants such as growth, flowering, development and fruiting. Water stress occurs directly or indirectly when plants are affected by abiotic factors such as drought, low temperature, high salinity, and heavy metals. In order to adapt to adversity, plants usually synthesize and accumulate a series of osmotic adjustment substances such as proline and betaine in a short period of time to enhance their resistance to osmotic stress. As an important osmotic regulator, proline (Pro) exists in plant cells in a free state, and participates in the process of plant stress resistance by regulating cell osmotic...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/26
Inventor 王晓冬王成任鹏胡叶何璐璐周航侯佩臣罗斌赵勇于春花
Owner BEIJING RES CENT OF INTELLIGENT EQUIP FOR AGRI
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