A kit for simultaneously detecting sodium and creatinine in urine

Abstract

The invention provides a kit simultaneously detecting sodium and creatinine in urine. The kit comprises a reaction base plate possessing a sodium reactive hole and a creatinine reactive hole, a sodium diluent, a creatinine diluent and a standard shade guide; a substrate pad and an enzyme pad are placed in the sodium reactive hole from bottom to top, the substrate pad is prepared by dropwise adding a substrate solution on a carrier, and the enzyme pad contains a beta-galactosidase and an enzyme stabilizing agent; an upper pad and a lower pad are placed in the creatinine reactive hole from bottom to top, the lower pad is prepared by dropwise adding an enzyme solution and a color developing agent on a carrier, and the lower pad contains an peroxidase, a creatinine amide hydrolase, a color developing agent, a stabilizing agent and the like; and the standard shade guide comprises standard color development of different concentration sodium ion and standard color development of different concentration creatinine. The kit preparation method is simple, the kit is stable in performance and easy to store, the color intervals corresponding to different concentration urine sodium and creatinine are obvious, whether the salt content of a detected person exceeds standard is convenient to determine, and also the kit is applicable to hospital clinical laboratory and non-professional examination personnel.

Description

technical field [0001] The invention relates to a method for measuring sodium and creatinine in urine by an enzymatic method, and a dry chemical kit for simultaneously detecting urine sodium and creatinine prepared by using the method, belonging to the technical field of medical inspection and measurement. Background technique [0002] Nephrogenic hypertension is the hypertension caused by kidney disease, which accounts for 5%-10% of adult hypertension, and is the most common secondary hypertension in the elderly. As one of the killers that endanger the health of the elderly, nephrogenic hypertension can cause vascular sclerosis, heart failure, cerebral hemorrhage, cerebral embolism, stroke, renal failure and other diseases, which seriously threaten the life and quality of life of the elderly. It has received more and more attention from the medical sector. [0003] At present, the detection methods of nephrogenic hypertension in China are relatively complicated, mainly inc...

AI Technical Summary

Problems solved by technology

[0008] At present, there is no dry chemical kit or similar method that can detect sodium and creatinine in urine at the same time. In order to meet the needs of grassroots testing institutions for non-quantitative detection of sodium and creatinine in urine and the lack of testing hardware for testing units , there is an urgent need to provide a fast, simple, semi-quantitative detection kit for semi-quantitative detection of sodium and creatinine in urine