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A kind of licorice asexual rapid propagation method

A licorice, rapid technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of affecting yield and quality, affecting licorice yield, low reproduction coefficient, etc., and achieve low equipment requirements, fast emergence and fast reproduction Effect

Active Publication Date: 2017-06-23
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The practice production propagation of Radix Glycyrrhizae adopts seed seedling raising and rhizome propagation usually, but seed germination rate generally only has 5-10% (1999 such as Yu Linqing), adopts rhizome segmentation to carry out rhizome propagation, and the amount of species is large, Affects the yield of licorice, and the reproduction coefficient is low. Long-term propagation with rhizomes is susceptible to viruses, leading to germplasm degradation, seriously affecting yield and quality

Method used

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  • A kind of licorice asexual rapid propagation method
  • A kind of licorice asexual rapid propagation method
  • A kind of licorice asexual rapid propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Asexual and rapid propagation of licorice uralensis

[0027] 1. Cultivation of sterile seedlings

[0028] Select and cut healthy and well-growing licorice twigs from the field about 15 cm long, cut off the leaves, rinse with tap water for 10 minutes, soak in a 0.1% carbendazim solution for 5-10 minutes, and then use tap water Wash 5 times. Cut the shoots into 5 cm long stems, soak them in 75% alcohol for 30 seconds, rinse them with sterile water once, use tweezers to remove the stems and place them in a 0.1% mercury solution for disinfection. Then rinse with sterile water for 6 times. Use sterile paper to absorb the moisture of the stems to obtain sterilized licorice shoots. Cut them into stem explants with 1 axillary bud and inoculate them one by one into the culture medium. , Placed at 22±1℃, light intensity 50μmol m -2 s -1 , Cultivate under the condition of light for 12h / day. After 7 days of culture, it can be seen that the stems are in contact with the m...

Embodiment 2

[0033] Example 2: Asexual and rapid propagation of different strains of licorice species

[0034] Using the same methods of cultivating, propagating and transplanting sterile seedlings as in Example 1, multiple strains such as Radix Glycyrrhizae Glabrae and Glycyrrhiza inflata Batalin have also achieved asexual and rapid propagation, with survival rates reaching up to More than 80%.

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Abstract

The invention discloses an asexual rapid propagation method for radix glycyrrhizae. The asexual rapid propagation method comprises the following steps: inoculating an explant of a radix glycyrrhizae stem section into a culture medium for cultivation to obtain an aseptic seedling; then cutting the aseptic seedling into a terminal bud explant, a stem section explant with one axillary bud and an explant with one axillary bud and a root, inoculating the explants into the same culture medium for cultivation to obtain a subculture-multiplicative aseptic seedling; and transplanting the subculture-multiplicative aseptic seedling into the culture medium to realize asexual rapid propagation for the radix glycyrrhizae. The formula of the used culture medium is that IBA with the concentration of 0.1mg / L is added to the MS culture medium, and the using amounts of KNO3 and NH4NO3 in the MS culture medium are halved respectively, and the pH value of the MS culture medium is 5.8. According to the asexual rapid propagation method, the root-inducing rate of the aseptic seedling is 100%, so that seedling emergence can be realized after 40 days, the seedling can be transplanted into a large field after 50 days, and the survival rate in the large field is higher than 80%. The asexual rapid propagation method can be suitable for rapid propagation of a plurality of excellent strain systems of a plurality of radix glycyrrhizae varieties, and has remarkable ecological, economic and social benefits in sustainable development of the radix glycyrrhizae.

Description

Technical field [0001] The invention belongs to the field of plant tissue culture, and specifically relates to a method for asexually and rapidly propagating licorice by using tissue culture technology. Background technique [0002] Glycyrrhiza uralensis Fisch (Glycyrrhiza uralensis Fisch) is a perennial herb belonging to the genus Glycyrrhiza in the leguminous family. It is distributed in the warm temperate and cold temperate continental monsoon climatic regions in the arid and semi-arid regions of northern my country. The altitude is 250-1400 meters and the annual average temperature is 3-10℃. . Licorice stems and leaves are good feed for livestock, and the roots have important economic value. It is the first bulk Chinese medicinal material among Chinese medicinal materials and a traditional bulk medicinal material for foreign exchange earning in my country. At the same time, licorice is also an important raw material in food, tobacco, cosmetics and other industries. . In order...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00
Inventor 吴国江李美茹
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI