Method for optimizing extraction technology of aquilaria sinensis leaf tea polyphenol by utilizing response surface process

A kind of agarwood leaf tea, response surface methodology technology, applied in medical preparations containing active ingredients, analysis by chemical reaction of materials, material analysis by observing the influence on chemical indicators, etc., can solve the problem of few researches. , waste of resources and other problems, to achieve the effect of high extraction rate, comprehensive utilization, and economic value improvement

Inactive Publication Date: 2016-02-24
NANCHANG UNIV
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Problems solved by technology

Nowadays, the utilization of agarwood is limited to only resin-containing agarwood wood, which can be used as medicine, and other parts such as leaves, seeds, branches, and woo

Method used

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  • Method for optimizing extraction technology of aquilaria sinensis leaf tea polyphenol by utilizing response surface process
  • Method for optimizing extraction technology of aquilaria sinensis leaf tea polyphenol by utilizing response surface process
  • Method for optimizing extraction technology of aquilaria sinensis leaf tea polyphenol by utilizing response surface process

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Embodiment 1

[0045] Embodiment 1: a kind of extraction technology utilizing response surface method to optimize the tea polyphenols of agarwood leaf, comprises the following steps:

[0046] 1) Preparation of test solution

[0047] Grind the agarwood leaves and put them in packaging bags for later use; accurately weigh 2.0g of the crushed agarwood leaves, and carry out water-bath leaching with an ethanol solution with a volume fraction of 30-70%. The leaching time is 10-50min, and the liquid-to-solid ratio is 20-40mL·g -1 , the extraction temperature is 50-90°C; after centrifuging the crude extract at a speed of 3500r / min for 6min, take the supernatant and add an equal volume of chloroform to the supernatant to mix and decolorize, stand in the dark for 1h and take The supernatant is the tea polyphenol mother liquor; pipette 1ml of the tea polyphenol mother liquor into a 100ml volumetric flask, dilute to the mark with deionized water, shake well, and wait for the test;

[0048] 2) Determin...

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Abstract

The invention provides a method for optimizing extraction technology of aquilaria sinensis leaf tea polyphenol by utilizing a response surface process. The method comprises the following steps: 1) preparing a test liquid; 2) determining the content of tea polyphenol in the extraction liquid; 3) performing experiment design and statistics analysis: a) performing single-factor experiment; and b) optimizing the design through the response surface process, concretely, taking the single-factor experiment as the basis, selecting extraction time, liquid-material ratio, extraction temperature and ethanol concentration as response variables, taking the aquilaria sinensis leaf tea polyphenol content as the response value, and employing the response surface process to optimize the aquilaria sinensis leaf tea polyphenol extraction conditions; and 4) analyzing and optimizing the experiment results, concretely, performing calculation on the regression equation through a Design-Expert. 8.05 software, so as to obtain the optimum technological conditions for extracting aquilaria sinensis leaf tea polyphenol.

Description

technical field [0001] The invention relates to an extraction process of tea polyphenols from agarwood leaves, in particular to an extraction process for optimizing tea polyphenols from agarwood leaves by using response surface method. Background technique [0002] Agarwood is the resinous wood of Aquilaria sinensis (Lour.) Gilg, a plant of the family Daphneaceae. It is a precious Chinese herbal medicine in my country. Hiccup, kidney deficiency and dyspnea. Nowadays, the utilization of agarwood is limited to resin-containing agarwood wood, which can be used as medicine, and other parts such as leaves, seeds, branches, and resin-free wood are all treated as waste, resulting in a large waste of resources. Agarwood leaves are the non-medicinal parts of Agaricus argillaceae, and there are few related studies. [0003] Tea polyphenols are active ingredients in tea, which have strong physiological activity and antioxidant properties. In addition, tea polyphenols also have a seri...

Claims

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Application Information

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IPC IPC(8): A61K36/835G01N21/78G01N21/33
CPCA61K36/835A61K2236/15A61K2236/333A61K2236/53G01N21/33G01N21/78
Inventor 潘军辉张国文谢雨婷李明智万宇俊喻芸
Owner NANCHANG UNIV
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