Method for preparing beta-chitin from sector bone of squid

A chitin and fan-shaped technology is applied in the field of chitin polysaccharide preparation, which can solve the problems of chitin degradation and serious environmental pollution, and achieve the effects of short time, good color and mild reaction conditions.

Active Publication Date: 2016-02-24
RONGCHENG GUANGRUN SEAFOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the shortcomings of serious environmental pollution and easy degradation of chitin in the extraction of chitin by the acid-base method in the prior art, the purpose of the present invention is to provide a utilization method with reasonable process, short preparation time, mild reaction conditions and less environmental pollution. Method for preparing β-chitin from squid fan-shaped bone

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for preparing beta-chitin by using squid fan-shaped bone, which goes through the following process steps:

[0021] (1) Raw material pretreatment: select fresh squid fan-shaped bone as raw material, dry at 55°C, grind to powder after drying, pass through a 5-mesh sieve for screening, and set aside;

[0022] (2) Ultrasound-assisted EDTA decalcification: Quantitatively weigh the squid fan-shaped bone powder obtained in step (1), add EDTA decalcification solution, the ratio of solid to liquid (mg / L) is 1:20, add concentrated hydrochloric acid, Adjust the pH value to 4.5, react in an ultrasonic water bath at 30°C for 30 minutes, and control the ultrasonic frequency at 50KHZ to obtain a reaction solution after decalcification; among them, EDTA decalcification is to add EDTA to warm distilled water at 65°C, stir, dissolve, and prepare Obtained; Among them, the ratio of EDTA to distilled water is 1:3 (m / v).

[0023] (3) Ultrasound-assisted acid compound protease depro...

Embodiment 2

[0028] A method for preparing beta-chitin by using squid fan-shaped bone, which goes through the following process steps:

[0029] (1) Raw material pretreatment: select fresh squid fan-shaped bone as raw material, dry at 60°C, grind to powder after drying, pass through a 10-mesh sieve for screening, and set aside;

[0030] (2) Ultrasound-assisted EDTA decalcification: Quantitatively weigh the squid fan-shaped bone powder obtained in step (1), add EDTA decalcification solution, the ratio of solid to liquid (mg / L) is 1:28, add concentrated hydrochloric acid, Adjust the pH value to 5.0, react in an ultrasonic water bath at 35°C for 20 minutes, and control the ultrasonic frequency at 40KHZ to obtain a reaction solution after decalcification; among them, EDTA decalcification is obtained by adding EDTA to 70°C distilled water, stirring, and dissolving; Among them, the ratio of EDTA to distilled water is 1:2 (m / v).

[0031] (3) Ultrasonic-assisted acidic compound protease deproteini...

Embodiment 3

[0036] A method for preparing beta-chitin by using squid fan-shaped bone, which goes through the following process steps:

[0037] (1) Raw material pretreatment: select fresh squid fan-shaped bones as raw material, dry at 40°C, grind to powder after drying, pass through a 3-mesh sieve for screening, and set aside;

[0038] (2) Ultrasound-assisted EDTA decalcification: Quantitatively weigh the squid fan-shaped bone powder obtained in step (1), add EDTA decalcification solution, the ratio of solid to liquid (mg / L) is 1:12, add concentrated hydrochloric acid, Adjust the pH value to 4.0, react in an ultrasonic water bath at 25°C for 50 minutes, and control the ultrasonic frequency at 71KHZ to obtain a reaction solution after decalcification; among them, EDTA decalcification is obtained by adding EDTA to distilled water at 60°C, stirring, and dissolving; Among them, the ratio of EDTA to distilled water is 1:4 (m / v).

[0039] (3) Ultrasound-assisted acid compound protease deprotein...

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Abstract

The invention discloses a method for preparing beta-chitin from the sector bone of a squid. The method mainly overcomes the problems of severe environmental pollution and great possibility of chitin degradation in extraction of chitin by using conventional acid-alkali methods. The method comprises the following steps: drying and crushing the sector bone of a squid, carrying out sieving, then adding EDTA decalcification solution and concentrated hydrochloric acid, adjusting a pH value and carrying out ultrasonic water bath so as to obtain a decalcified reaction solution; adding acidic composite protease into the reaction solution, carrying out ultrasonic-assisted enzymatic hydrolysis and filtering and washing filter residue until the filter residue is neutral; and soaking the filter residue with a hydrochloric acid solution and carrying out secondary decalcification and drying so as to obtain a beta-chitin product. The method provided by the invention employs an ultrasonic-assisted EDTA process and enzyme process to prepare beta-chitin and has the advantages of short time, simple operation, mild conditions, small pollution to the environment and no influence on the structure of beta-chitin; and prepared beta-chitin has high purity and a good color.

Description

technical field [0001] The invention relates to the preparation of chitin polysaccharide, in particular to a method for preparing beta-chitin by using squid fan-shaped bones. Background technique [0002] Chitin, also known as chitin, chitin, etc., is a glucose-based branched polysaccharide and is the second most abundant biopolymer in nature. Chitin can remove the "garbage" in the human body to achieve the purpose of preventing diseases and prolonging life. It is currently widely used in many fields such as agriculture, medicine, daily chemical industry, and food processing. In nature, chitin exists in three crystal forms, which are divided into α-, β-, and γ-three types, among which α-chitin exists most widely, and β-chitin is rare. Chitin extracted from the fan-shaped bone of squid is β-chitin. Compared with α-chitin, β-chitin has better biodegradability, aggregation activity and easy microfibrillation. [0003] Chitin widely exists in the shells of crustaceans (shrimp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 杨劲峰吴文惠高凯莉包斌
Owner RONGCHENG GUANGRUN SEAFOOD CO LTD
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