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Analytical method of phytoplankton particle size structure

A technology for phytoplankton and structure analysis, which is applied in the field of phytoplankton particle structure analysis, and can solve the problems of large consumption of reagents, inability to indicate the particle size of a single algal cell, and cumbersome processing.

Inactive Publication Date: 2017-12-19
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, not only can the phytoplankton community composition be accurately determined at the phylum level, but also some species can be accurately identified and determined; however, the pigment analysis method requires high-performance liquid chromatography, which consumes a lot of reagents and cumbersome sample pretreatment It is worth noting that this method does not strictly reflect the true particle size of the phytoplankton community, and some diagnostic pigments may be in multiple phytoplankton communities at the same time; therefore, the results calculated by this method have stronger statistical significance, It cannot indicate the particle size of individual algae cells
[0013] The measurement of phytoplankton particle size structure using the above method is limited to the collected water samples, so there are still many limitations on the degree of spatial coverage on horizontal or vertical scales

Method used

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  • Analytical method of phytoplankton particle size structure

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Effect test

Embodiment 1

[0095] A method for analyzing the particle size structure of seawater phytoplankton, comprising the following steps:

[0096] 1) In-situ measurement of seawater absorption spectrum:

[0097] Use water body light attenuation and absorption parameter measuring instruments, such as figure 1 As shown, it is mainly composed of a light-emitting unit 1, a sampling unit 2, a detection unit 3, and a signal acquisition and analysis unit 4; the light-emitting unit 1 includes a main channel 11 and three sub-channels divided into one end of the main channel 11, which are respectively defined as absorption channels 12 , the attenuation channel 13 and the reference channel 14, the main channel 11 and the absorption channel 12 are directly connected, and the attenuation channel 13 is directly connected with the reference channel 14;

[0098] Such as figure 2 As shown, the main channel 11 is provided with a condenser cover 111, a light source 112 for sampling a high-stable tungsten halogen ...

Embodiment 2

[0125] A method for analyzing the particle size structure of seawater phytoplankton, comprising the following steps:

[0126] 1) In-situ measurement of seawater absorption spectrum:

[0127] Concrete measurement method is with embodiment 1;

[0128] 2) extract phytoplankton absorption coefficient from the spectral absorption coefficient a (λ) of described seawater by decomposition method:

[0129] According to the existing research, the spectral absorption coefficient a(λ) of seawater is expressed according to the composition of the water body as:

[0130] a(λ)=a w (λ)+a p (λ)+a CDOM (λ) (14)

[0131] Among them, a w (λ), a p (λ) and a CDOM (λ) represent the absorption coefficients of pure seawater (w), total particulate matter (p) and colored dissolved organic matter (CDOM), respectively;

[0132] The absorption coefficient of total particulate matter (p) can further decompose the absorption coefficient of phytoplankton (ph) a ph (λ) and non-algae particulate matter...

Embodiment 3

[0148] A method for analyzing the particle size structure of seawater phytoplankton, comprising the following steps:

[0149] 1) In-situ measurement of seawater absorption spectrum:

[0150] Concrete measurement method is with embodiment 1;

[0151] 2) extract phytoplankton absorption coefficient from the spectral absorption coefficient a (λ) of described seawater by decomposition method:

[0152] Concrete extraction method is with embodiment 2;

[0153] 3) Extract the size parameter S of phytoplankton based on the shape change of phytoplankton absorption spectrum :

[0154] First, according to the effect of algae particle size on the shape change of phytoplankton absorption spectrum, a hybrid spectral model is established, and the particle size parameter S :

[0155]

[0156] in: is the normalized absorption coefficient of phytoplankton, and represent the normalized absorption coefficients of picophytoplankton and microphytoplankton, respectively, and the pa...

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Abstract

The invention provides a phytoplankton particle size structure analysis method. After measuring the seawater absorption spectrum in situ, the phytoplankton absorption spectrum contribution is extracted through a decomposition method, and a bio-optical inversion algorithm is used to further extract the average particle size of algae and phytoplankton. Particle-level structural information. The method of the invention can realize in-situ, high-frequency and high-vertical resolution measurement, and is of great significance for quickly identifying the particle size structure and spatial and temporal distribution of phytoplankton biomass.

Description

technical field [0001] The invention relates to a method for analyzing water body conditions, in particular to a method for analyzing the granular structure of phytoplankton in seawater. Background technique [0002] Phytoplankton refers to tiny plants that live on plankton in water. Usually, phytoplankton refers to phytoplankton algae, including diatoms, dinoflagellates, green algae, cyanobacteria, golden algae, yellow algae, etc., as well as autotrophic bacteria. Phytoplankton are widely distributed on the earth and are the most important primary producers in aquatic ecosystems. They start the food web of aquatic ecosystems and play a vital role in energy flow, material circulation and information transmission. [0003] The study on the particle size structure of phytoplankton is of great significance to understand the functions of phytoplankton with different particle sizes in the ecosystem. The particle size classification of phytoplankton generally refers to the standar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/02
CPCG01N15/0205G01N15/01
Inventor 周雯孙兆华邹国旺许占堂王桂芬李彩杨跃忠曹文熙
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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