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Melanin-containing in vitro skin test model and preparation method

A skin model and in vitro testing technology, applied in biological testing, measuring devices, material testing products, etc., can solve the problems of poor melanin model culture stability, can not simulate the structure of natural skin basal layer well, and achieve good differentiation structure, Promoting long-term survival, tight dermal junction effects

Active Publication Date: 2016-02-24
GUANGDONG BOXI BIO TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] It can be seen from the above analysis that although relevant melanin models have been developed, the basal layer of these models is mainly derived from exogenous extracellular matrix or inert carrier, and such a basal layer cannot simulate natural skin well. The structure of the basal layer of the melanin model, so there are certain limitations in the later development process, which eventually leads to the poor stability of the later culture of the melanin model

Method used

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  • Melanin-containing in vitro skin test model and preparation method
  • Melanin-containing in vitro skin test model and preparation method
  • Melanin-containing in vitro skin test model and preparation method

Examples

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Embodiment 1

[0037] Example 1 A kind of in vitro skin test model containing melanin and its preparation method

[0038] The prepared skin test model mainly includes the dermis and the epidermis, with a microporous polyester cellulose membrane as the supporting membrane, including: the dermis containing multiple layers of active fibroblasts and secreted extracellular matrix and the melanin-containing The epidermis of cells is constructed.

[0039] The preparation method of the skin test model of the present embodiment is as follows with the following steps:

[0040] 1. Acquisition of cells used in construction Acquisition of cells used in construction

[0041] 1. Acquisition and subculture of primary melanocytes: Melanocytes can be derived from circumcised normal skin tissue. For details on the acquisition and subculture, please refer to Liu Yuan’s "Research on the Construction of Tissue Engineering Skin Containing Melanocytes" (results in the appendix figure 1 , 2 , 3);

[0042] 2....

Embodiment 2

[0052] Example 2 A kind of in vitro skin test model containing melanin and its preparation method

[0053] The skin test model of this embodiment is similar to that of Embodiment 2. Its preparation method steps are as follows:

[0054] 1. Acquisition of cells used in construction

[0055] 1. Acquisition and subculture of primary melanocytes: Melanocytes can be derived from circumcised normal skin tissue. For details on the acquisition and subculture, please refer to Liu Yuan’s "Research on the Construction of Tissue Engineering Skin Containing Melanocytes" (results in the appendix figure 1 , 2 , 3);

[0056] 2. Acquisition and subculture of keratinocytes: Keratinocytes can be derived from normal skin tissue after circumcision. For details on the acquisition and subculture, please refer to "Isolation, Purification, Culture and Identification of Human Epidermal Stem Cells" by Ma Yingzhi;

[0057] 3. Acquisition and passage of fibroblasts: Fibroblasts can be derived from ...

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Abstract

The present invention discloses a melanin-containing skin models in vitro skin test model and a preparation method and use. The test model comprises a dermis layer and an epidermis layer, the epidermis layer comprises epidermal cells and melanin cells, and the dermis layer is a membrane comprising fibroblast and an extracellular matrix formed by the fibroblast. The skin model is very similar to the human skin structure, the building process simulates the skin development process, the epidermis layer can be developed under nourishing of the dermis layer, and synthesis, secretion, transport and the like of melanin can be promoted. The present invention discloses a preparation method and use of the skin test model in whitening and freckle removal effect testing of a variety of cosmetics, health products and pharmaceuticals, related mechanism exploration and research, and the like.

Description

technical field [0001] The invention belongs to the field of tissue engineering biomaterials, in particular to an in vitro skin test model containing melanin and a preparation method thereof. Background technique [0002] Cosmetics, pharmaceuticals, chemicals and other products must undergo a large number of tests before they go on the market to ensure the safety and efficacy of the products. Traditional detection is generally carried out through animal experiments, but due to the deviation of animal experiments and the proposal of the 3R theory, researchers are trying to find more suitable detection methods. At present, a variety of skin models have been constructed based on the principles of tissue engineering, which are used to replace animal models to study different biological functions of the skin, as well as the skin's response to cosmetic or pharmaceutical preparations. A good skin model should have high consistency with human skin test results. [0003] In the saf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5008
Inventor 程蕊苹卢永波李潇
Owner GUANGDONG BOXI BIO TECH CO LTD
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