Urea detection kit

A technology for detection kits and reagents, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of waste, poor accuracy, and influence on the stability of reagents, and achieves enhanced stability, increased stability time, Excellent stability

Inactive Publication Date: 2016-03-02
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the presence of enzymes in ordinary urea detection reagents, the stability of the reagent will be affected, which is not conducive to the long-term storage of the reagent, resulting in poor accuracy and waste.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A urea kit with excellent accuracy recognized on the market, which includes reagent R1, reagent R2, and calibrator.

[0037] Wherein the reagent R1 consists of:

[0038] Tris buffer 100mmol / L

[0039] α-ketoglutarate 8mmol / L

[0040] Glutamate dehydrogenase 1000U / L

[0041] Sodium azide 0.5% (W / V)

[0042] Reagent R2 consists of:

[0043] Tris buffer 100mmol / L

[0044] Urease 2000U / L

[0045] NADH0.8mmol / L

[0046] The test kit described in this embodiment, when in use, its assay method is to adopt the Toshiba 120 automatic analyzer with double reagent function, operate as follows:

[0047] Add 4 μl of physiological saline, sample or calibrator, then add 300 μl of R1 reagent for pre-incubation for 5 minutes, then add 100 μl of reagent R2, start recording the absorbance value A1 after 30 seconds, and read the absorbance A2 after 5 minutes of reaction, and calculate ΔA / min.

[0048] The calibrator used in this embodiment is a composite calibrator produced by Landa...

Embodiment 2

[0050] A urea detection kit includes reagent R1, reagent R2 and calibration products.

[0051] Wherein the reagent R1 consists of:

[0052] Tris buffer 100mmol / L

[0053] α-ketoglutarate 8mmol / L

[0054] Glutamate dehydrogenase 1000U / L

[0055] Sodium azide 0.5% (W / V)

[0056] Reagent R2 consists of:

[0057] Tris buffer 100mmol / L

[0058] Urease 2000U / L

[0059] NADH0.8mmol / L

[0060] 4-FPBA0.01% (W / V)

[0061] Glucomannan 0.5mg / mL

[0062] NaCl5mmol / L

[0063] Propylene glycol 20mg / mL

[0064] Triton 1001% (V / V)

[0065] BSA0.1g / L

[0066] Concrete determination method is with embodiment 1.

Embodiment 3

[0068] A urea detection kit includes reagent R1, reagent R2 and calibration products.

[0069] Wherein the reagent R1 consists of:

[0070] Tris buffer 100mmol / L

[0071] α-ketoglutarate 8mmol / L

[0072] Glutamate dehydrogenase 1000U / L

[0073] Sodium azide 0.5% (W / V)

[0074] Reagent R2 consists of:

[0075] Tris buffer 100mmol / L

[0076] Urease 2000U / L

[0077] NADH0.8mmol / L

[0078] 4-FPBA0.01% (W / V)

[0079] Glucomannan 0.8mg / mL

[0080] NaCl5mmol / L

[0081] Propylene glycol 20mg / mL

[0082] Triton 1001% (V / V)

[0083] BSA0.5g / L

[0084] Concrete determination method is with embodiment 1.

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Abstract

The invention discloses a urea detection kit, and belongs to the technical field of clinical in-vitro detection reagents. The kit comprises a reagent R1, a reagent R2 and a calibration material. A compound stabilizer composed of 4-FPBA, glucomannan, NaCl, propylene glycol, triton X-100 and BSA is added in the reagent R2, so that the stability of the kit is effectively improved, the linearity range is relatively good, the reagent accuracy is high, and further popularization and application in the market are facilitated.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to a urea detection kit. Background technique [0002] Urea is an organic compound composed of carbon, nitrogen, oxygen and hydrogen, also known as urea (same as urine). Its chemical formula is CON 2 h 4 , (NH 2 ) 2 CO or CN 2 h 4 O, molecular weight 60, international non-proprietary drug name is Carbamide. Appearance is white crystal or powder. It is a product of animal protein metabolism and is usually used as nitrogen fertilizer for plants. Urea is synthesized in the liver and is a nitrogenous metabolite excreted by mammals. This metabolic process is called the urea cycle. [0003] Urea (UREA) is the final product of amino acid metabolism in the human body. It constitutes most of the non-protein nitrogen in the blood. It is synthesized in the liver and excreted into the urine through the kidneys. Therefore, its content depends on protein int...

Claims

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Application Information

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IPC IPC(8): C12Q1/58C12Q1/34C12Q1/32
CPCC12Q1/58C12Q1/32
Inventor 董雯甘宜梧李静
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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