Genetic marker assisted selection method for increasing Canadian large white pig IMF

A genetic marker and assisted selection technology, applied in the field of genetic marker assisted selection, to achieve the effects of being conducive to early diagnosis, strong genetic stability, and improving flavor and tenderness

Inactive Publication Date: 2016-03-02
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the improvement of breeding technology, more and more defects have been exposed in the past relying on a single gene as a molecular marker for IMF selection

Method used

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  • Genetic marker assisted selection method for increasing Canadian large white pig IMF
  • Genetic marker assisted selection method for increasing Canadian large white pig IMF
  • Genetic marker assisted selection method for increasing Canadian large white pig IMF

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Take 50 g of longissimus dorsi, autoclave at 121°C for 30 minutes, dry at 133°C for 90 minutes, pulverize, and filter through a 100-mesh sieve. Take 50 mg of the above sample and add 320 μL of buffer solution, shake to suspend. Add 20 μL proteinase K solution, bathe in water at 65°C for 20 minutes, and mix 3 times. Add 340 μL buffer GB and bathe in water at 65°C for 10 minutes. Centrifuge for 2 minutes. Take 440 μL of supernatant, add 220 μL of water and ethanol, and mix 8 times to obtain a flocculent precipitate and solution. Pour all the above flocculent precipitate and solution into the adsorption column CB3, and put it in a collection tube, centrifuge for 2 minutes, pour off the waste liquid; add 500 μL buffer GD, centrifuge for 30 seconds, pour off the waste liquid; add 700 μL rinse solution Pw , centrifuge for 30s, pour off the waste liquid; add 500μL rinse solution, centrifuge for 30s, pour off the waste liquid. Place the adsorption column CB3 at room tempera...

Embodiment 2

[0048] Take 50 g of longissimus dorsi, autoclave at 121°C for 30 minutes, dry at 133°C for 90 minutes, pulverize, and filter through a 100-mesh sieve. Take 50 mg of the above sample and add 320 μL of buffer solution, shake to suspend. Add 20 μL proteinase K solution, bathe in 65°C water for 30 minutes, and mix 3 times. Add 340 μL buffer GB and bathe in water at 65°C for 10 minutes. Centrifuge for 2 minutes. Take 440 μL of supernatant, add 220 μL of water and ethanol, invert and mix 7 times to obtain flocculent precipitate and solution. Pour all the above flocculent precipitate and solution into the adsorption column CB3, and put it in a collection tube, centrifuge for 2 minutes, pour off the waste liquid; add 500 μL buffer GD, centrifuge for 30 seconds, pour off the waste liquid; add 700 μL rinse solution Pw , centrifuge for 30s, pour off the waste liquid; add 500μL rinse solution, centrifuge for 30s, pour off the waste liquid. Place the adsorption column CB3 at room tempe...

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Abstract

The invention provides a genetic marker assisted selection method for increasing Canadian large white pig IMF. The method comprises the following steps: taking peroxysome proliferator-activated receptor PPAR gamma and sterol regulatory element-binding protein ADD1 genes as molecular marker genes; drying and sieving longissimus dorsi; successively adding a buffer solution, a protease K solution, a buffer solution GB and ethyl alcohol, respectively mixing evenly, carrying out water bath, and centrifuging to obtain flocculent precipitation and a solution; placing a product in an adsorption column, rinsing, eluting and centrifuging; respectively carrying out amplification on fragments of a gene exon coding region according to PPAR gamma and ADD1 primers, purifying and recycling, carrying out sequence determination, analyzing polymorphic sites and genotypes, determining AA-CT-TC genotypes of PPAR gamma and ADD1 and the AA-CT-TC genotypes as a molecular genetics assisted marker for increasing IMF. By the method, Canadian large white pig IMF breeding stereotaxis and high efficiency can be improved, so that flavor and tenderness of meat are improved. Single nucleotide polymorphism SNP of genes can be accurately detected, genetic typing detection accuracy is close to 100%, meanwhile, the method can be used for detecting living bodies, and early diagnosis is facilitated.

Description

technical field [0001] The invention belongs to a genetic marker-assisted selection method, in particular to a genetic marker-assisted selection method for increasing the intramuscular fat content (IMF) of a large white pig. Background technique [0002] The large white pig, also known as the Great Yorkshire, originated in the United Kingdom. Because of its large size, strong reproductive ability, high feed conversion rate and slaughter rate, and strong adaptability, Large White pigs are raised in all countries with developed pig farming industries in the world. It is the most famous and widely distributed leading lean pig breed in the world. . Since it was introduced into my country in the 1950s, the Large White pig has become the most widely used lean-meat pig breed. Although large white pigs have a high lean meat percentage, with the continuous improvement of people's living standards, people's requirements for pork quality are also getting higher and higher. Intramusc...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 刘显军边连全陈静张铁赢
Owner SHENYANG AGRI UNIV
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