Breeding and cultivating method of nelumbo nucifera by using rhizome of lotus in test tube as material

A cultivation method and test tube lotus root technology are applied in the field of agricultural and horticultural cultivation, which can solve problems such as lack of methods and achieve the effect of solving the shortage of varieties.

Inactive Publication Date: 2016-03-09
SUZHOU ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And for the propagation and cultivation of Bowl Lotus, especially the miniaturiz

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0024] Taking Suxiu, a large variety of lotus, as an example, after induced test-tube lotus root and hardening, transplanting and planting of test-tube lotus root, the flowering period management and cultivation were finally carried out.

[0025] Wherein, inducing test tube lotus root, specifically comprises the following steps:

[0026] (1) Shoot apex priming: select terminal buds or side buds of disease-free Hualien as explants, peel off the outer leaf sheath, keep 1 leaf and 1 mixed bud, inoculate on lotus root stem apex priming medium and cultivate until it turns green, Among them, the shoot tip starting medium is 1 / 2MS+6-BA1.5mg / L+NAA0.1mg / L+4.0% sucrose+agar 0.7%;

[0027] (2) Inducing cluster buds and proliferation: transfer the green shoot tips to the cluster bud induction medium, and after the cluster buds are produced, the single buds or cluster buds will be cut and transferred to the induction medium for subculture and multiplication culture. Until new cluster buds...

Embodiment 2

[0034] Taking Shuiyue, a medium-sized lotus variety, as an example, after induced test-tube lotus root and test-tube lotus root hardening, hydroponics, and finally management and cultivation of the flowering stage of breeding.

[0035] Wherein, inducing test tube lotus root, specifically comprises the following steps:

[0036] (1) Shoot apex priming: select terminal buds or side buds of disease-free Hualien as explants, peel off the outer leaf sheath, keep 1 leaf and 1 mixed bud, inoculate on lotus root stem apex priming medium and cultivate until it turns green, Among them, the shoot tip starting medium is 1 / 2MS+6-BA1.0mg / L+NAA0.2mg / L+4.0% sucrose+agar 0.7%;

[0037] (2) Inducing cluster buds and proliferation: transfer the green shoot tips to the cluster bud induction medium, and after the cluster buds are produced, the single buds or cluster buds will be cut and transferred to the induction medium for subculture and multiplication culture. Until new cluster buds are produc...

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PUM

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Abstract

The invention provides a breeding and cultivating method of nelumbo nucifera by using rhizome of lotus in a test tube as a material. The breeding and cultivating method comprises the steps of inducing the rhizome of lotus in the test tube, and performing hardening seedling, transplanting, field planting and subsequent growth period management on the rhizome of lotus in the test tube. The method provided by the invention can be directly applied to nelumbo nucifera bonsai cultivation; by establishing a corresponding cultivation method, plants can be miniaturized, and the aim that the vast majority of lotus varieties are suitable for bonsai and ornament is achieved, so that the problem that the current bonsai small-sized bowl lotus varieties are insufficient is solved.

Description

technical field [0001] The invention relates to the technical field of agricultural and horticultural cultivation, in particular to a method for propagating and cultivating bowl lotus. Background technique [0002] Wanlian refers to a type of miniature lotus that can grow and bloom normally in pots with a diameter of less than 26cm. At present, there are more than 30 lotus varieties (lines) that can be successfully cultivated in pots, but small plant varieties that can truly express stably There are no more than 10, and the bowl lotus is mainly Asian lotus, and its variation is mainly limited to a few limited phenotypic traits. [0003] Wanlian likes strong light, which is good for its growth. It also likes water and is afraid of flooding, deep water is easy to rot leaves, likes fertilizer but is not tolerant to fertilizer, heavy fertilizer is easy to rot stems, the soil is not strict, likes fertile slightly acidic clay soil, generally fertile river mud or vegetable garden ...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G1/00
CPCA01G22/00A01H4/008
Inventor 徐君李静会李欣江君姜红卫
Owner SUZHOU ACADEMY OF AGRI SCI
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