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Rapid propagation method of lonicera oblata

A clove and rapid technology, applied in the field of aseptic rapid propagation of clove leaf honeysuckle, can solve problems such as difficulties in the rapid propagation system, and achieve the effects of improving the survival rate of inoculation, easily inducing cluster buds, and growing vigorously.

Inactive Publication Date: 2016-03-16
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is extremely difficult to establish an aseptic rapid propagation system for Lonicera cloves

Method used

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  • Rapid propagation method of lonicera oblata
  • Rapid propagation method of lonicera oblata
  • Rapid propagation method of lonicera oblata

Examples

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Embodiment 1

[0035] According to the rapid propagation method of clove leaf honeysuckle of the present invention, according to the following steps, carry out the rapid propagation of clove leaf honeysuckle:

[0036] 1. Collect branches

[0037] The used explant of the present invention is the clove leaf honeysuckle bud body with trace xylem, thereby, will gather material according to following requirement:

[0038] 1.1 Collection time: The best time is from late August to early September, which is fuller than winter and spring, and there are more living cells in the cortex of branches, which are easy to peel off.

[0039] 1.2 Collection method: from 12:00 noon to 2:00 p.m., collect on wild clove-leaf honeysuckle plants, cut annual branches with sharp scissors, and use a pair of clean small scissors to quickly cut off the leaves from the middle and lower part of the petiole, and keep a small section The petioles are used to protect the axillary buds, and at the same time, pay special atten...

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Abstract

The invention discloses a rapid propagation method of lonicera oblata. The method comprises the steps that 1, an explant is prepared, wherein a lonicera oblata bud with a trace amount of xylem is taken, bud scales are stripped through a single-sided blade to enable the bud to have the xylem of which the thickness is not larger than 0.5 mm, and the treated bud serves as the explant; 2, disinfection is conducted, wherein in a super clean bench, the explant obtained through cutting is transferred into a triangular flask containing a 10% fresh hydrogen peroxide solution through autoclaved tweezers, 3-5 drops of tween-60 is dropwise added, oscillation is conducted for 12 min, the hydrogen peroxide solution is poured out, the explant is washed with sterile water, the water is leached and drained, and inoculation is prepared; 3, inoculation is conducted, wherein the disinfected explant is transferred into a culture flask containing a proliferation starting medium for closed culture; 4, rooting culture is conducted; 5, acclimatization and transplanting are conducted on a rooting seedling. By means of the rapid propagation method of the lonicera oblata, rapid propagation of the lonicera oblata can be effectively conducted, the contamination rate is low, and the inoculation survival rate and the cluster bud induction rate are high.

Description

technical field [0001] The invention relates to a method for rapid aseptic propagation of clove-leaf honeysuckle. Background technique [0002] Lilac leaf honeysuckle is a second-class endangered plant in Beijing. There are 7 plants recorded in the Beijing area, but only one plant has been seen. In order to save this endangered plant, the number of individuals in its population should be expanded first, but no seeds have been collected for many years, and a certain number of individual plants can only be obtained by exploring the means of tissue culture and rapid propagation, and using the established rapid propagation technology of clove leaf honeysuckle The provenance of the surrounding provinces and regions is propagated, and then re-cultivated into the wild environment together with the local provenance of Beijing to realize the return of clove-leaf honeysuckle to the wild. However, it is extremely difficult to establish an aseptic rapid propagation system for Lonicera ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 任建武卢宝明蒋万杰吴记贵
Owner BEIJING FORESTRY UNIVERSITY