Method for artificial inoculation of Tilletia foetida infected wheat

Abstract

The invention provides a method for artificial inoculation of Tilletia foetida infected wheat and belongs to the field of agricultural microbiology. The method includes: (1), preparing a Tilletia foetida hypha suspension for infection, namely crushing mycocecidium of Tilletia foetida, preparing a spore suspension, coating the spore suspension on a 2%water agar medium, placing the water agar medium in an incubator at 16 DEG C for incubation until teliospores germinate and hyphae grow out, and using sterilized distilled water to flush down the hyphae for standby use; (2), preparing for inoculation of wheat seedlings, namely soaking wheat seeds until sprouting, subjecting the wheat seeds to vernalization at 5 DEG C for one month, planting the vernalized seeds in sterilized soil, and taking the wheat seedlings when they grow to a jointing-booting stage for standby use; (3), inoculating, namely injecting the Tilletia foetida hypha suspension obtained in the step (1) into a gap of pollen chambers of wheat plants. By adopting the method, success rate of obtaining diseased wheat plants is higher. The method is simple and easy to implement, convenient to implement and operate and conducive to popularization and application.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to a method for artificially inoculating wheat with Tilletia glabrata. Background technique [0002] Tilletia tritici, also known as Tilletia tritici (Latin name: Tilletiafoetida), belongs to the order Tilletiaceae, Tilletiaceae, and the genus Tilletia tritici. It can cause tickle smut in wheat. When the teliospores of T. tritici germinated, only one original hyphae was produced, but the inner wall was disintegrated in many places. The original mycelium contains multiple bacterial nuclei, and there are fat particles, ribosomes, glycogen and mitochondria. Eight basidiospores can be formed on the top of the original hyphae, and bridged to form "H" type hyphae. Its spores are born in the ovary, surrounded by pericarp, and are the same size as the seeds. Its spores are spherical to oval, sometimes oblong to polygonal, light grayish brown to olive brown, smooth,...

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Problems solved by technology

[0003] In the research on the physiological growth and infection mechanism of T. tritici, it is usually necessary to inoculate it into wheat plants by artificial means so that the latter can infect the infected wheat plants for observation and analysis, but so far, no There is an effective method of artificially inoculating wheat with Tilletia tritici to obtain wheat plants with high incidence for research

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