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Method for extracting protein from low-temperature rice bran meal

A rice bran meal and protein technology, which is applied in the field of protein extraction technology, can solve the problems of insignificant effect of physical methods, unfavorable industrial production, and high enzyme price, and achieve the effects of promoting development and utilization, alleviating the shortage of protein resources, and improving the quality of rice bran protein

Inactive Publication Date: 2016-04-13
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzymatic extraction can effectively improve the extraction rate of protein, and it is simple and fast, but the disadvantage is that the price of enzyme is too high, which is not conducive to industrial production
But the effect of physical method is not significant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] After passing through 60-100 meshes of low-temperature rice bran meal, add sodium hydroxide alkali solution (pH value 9.0) to extract for 1 hour, and then centrifuge to obtain protein extract (protein extraction rate is 50%). The pH value of the obtained protein extract is adjusted to 4.0-4.5 to precipitate protein, and the obtained protein precipitate is redissolved and dried to obtain rice bran protein powder.

Embodiment 2

[0028] After the low-temperature rice bran meal passes through 60-100 meshes, mix it with deionized water at a ratio of 1:10, immerse the ultrasonic probe in the low-temperature rice bran meal suspension, and perform ultrasonic treatment under the conditions of ultrasonic power 500W and ultrasonic time 30 minutes. After the ultrasonic treatment, adjust the pH value of the low-temperature rice bran meal suspension to 9.0 with sodium hydroxide solution, extract for 1 hour, and centrifuge to obtain a protein extract (56% protein extraction rate), and adjust the pH value of the obtained protein extract to 4.0~ 4.5 To precipitate protein, the obtained protein precipitate can be obtained rice bran protein powder after reconstitution and drying.

Embodiment 3

[0030] After the low-temperature rice bran meal passes through 60-100 mesh, it is mixed evenly with 0.6% dilute sulfuric acid at a ratio of 1:10, and soaked at 90°C for 3 hours. After soaking in dilute acid, immerse the ultrasonic probe in the prepared low-temperature rice bran meal suspension, perform ultrasonic treatment under the conditions of ultrasonic power 300W, ultrasonic time 30min, and then centrifuge at 3000rpm-10000rpm for 10min to obtain the protein extract (the Under acidic conditions, the protein extraction rate is 22%), the resulting extract is adjusted to a pH value of 4.0 to 4.5 to precipitate protein, and the centrifuged solid meal is added to a sodium hydroxide alkaline solution (pH value 9.0) to extract for 1 h, and then centrifuged to obtain a protein extract ( The protein extraction rate was 40%), and the pH value was adjusted to 3.5-4.5 to precipitate the protein, and the precipitated protein was collected twice to obtain rice bran protein powder after b...

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Abstract

The invention discloses a method for extracting protein from low-temperature rice bran meal. The method comprises the following steps: 1) immersing low-temperature rice bran meal in a diluted acid, crushing and sieving through a sieve of 60-100 mesh; 2) performing ultrasonic processing; 3) performing centrifugation separation to respectively obtain a supernatant and a solid meal; 4) precipitating rice bran protein from the supernatant; 5) processing the solid meal with a diluted alkali solution, performing centrifugation separation and protein precipitation; and 6) performing drying processing. According to the method, sulfuric acid is utilized for processing low-temperature rice bran meal through immersing, so that protein in the low-temperature rice bran meal is at a molten globule state, and through ultrasonic processing, depolymerization and dissolving of rice bran protein are promoted, and solubility of rice bran protein under an acid condition and the rice bran protein extraction rate are effectively improved. The method is mild in preparation conditions, high in efficiency and low in cost, and the extracted rice bran protein is good in quality.

Description

technical field [0001] The invention relates to a protein extraction process, in particular to a method for extracting protein from low-temperature rice bran dregs. Background technique [0002] my country is an important rice producing country in the world. Rice bran is the most important by-product in rice processing, with an annual output of more than 10 million tons. However, there are still deficiencies in the deep processing and comprehensive utilization of rice bran in my country, with only 10 %~15% of the rice bran is used for deep processing, most of the rice bran is mainly used as feed, and the resources have not been rationally developed and utilized. [0003] Rice bran contains 15% to 24% oil and 12% to 18% protein, of which rice bran protein is mainly composed of albumin, globulin, glutenin and gliadin . Studies have shown that the essential amino acids in rice bran protein are complete, and the composition and ratio of amino acids are close to the recommended ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/14
Inventor 张维农张燕鹏齐玉堂胡志雄孙晓梅祝贤彬
Owner WUHAN POLYTECHNIC UNIVERSITY
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