A kind of wheat anti-ear germination gene tazfp18 and its application

A technology for ear germination and wheat, which is applied to the wheat ear germination resistance gene TaZFP18 and its application fields, and can solve the problems affecting the efficiency and accuracy of molecular marker-assisted breeding selection.

Active Publication Date: 2019-01-04
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the identified QTL is not a functional marker, it only represents a certain segment on the chromosome, which affects the efficiency and accuracy of molecular marker-assisted breeding selection

Method used

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  • A kind of wheat anti-ear germination gene tazfp18 and its application
  • A kind of wheat anti-ear germination gene tazfp18 and its application
  • A kind of wheat anti-ear germination gene tazfp18 and its application

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Embodiment 1

[0019] 1. Materials

[0020] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified. The reagents and other materials used are all commercially available products unless otherwise specified. The germplasm resources used can all be obtained from national species The quality resource library is obtained.

[0021] 2. Method

[0022] 2.1 Extraction of wheat genomic DNA by SDS-Tris saturated phenol method

[0023] (1) Take 2-3 seeds of 8 kinds of wheat varieties respectively, place them in 2ml sterilized centrifuge tubes, and use a high-throughput tissue grinder to grind them into powder. The wheat varieties are Hongmangchun 21, Jing 411, Suining Tuotuo wheat, Jimai 22, Fuling Xuxu wheat, Yannong 19, Yongchuan white shell wheat, Zhongyou 9507, of which Jing 411, Jimai 22, Yannong 19, Zhongyou 9507 can be obtained from the market, Hongmanshun 21 , Suining Tuotuo wheat, Fuling Xuxu wheat, Yongchuan white shell wheat (...

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Abstract

The invention discloses a wheat pre-harvest sprouting tolerance gene TaZFP18. The gene includes the nucleotide sequence shown as SEQ ID NO.1, or includes the nucleotide sequence shown as complementary with the SEQ ID NO.1 sequence, and the gene encodes a CCCH type zinc finger protein 18 and can serve as the molecular marker gene of a wheat pre-harvest sprouting tolerance variety. The wheat pre-harvest sprouting tolerance gene TaZFP18 is a novel major wheat pre-harvest sprouting tolerance gene, can be utilized to develop a molecular marker so as to rapidly identify whether wheat belongs to the pre-harvest sprouting tolerance variety or not and provides a new gene resources for breeding of wheat pre-harvest sprouting tolerance varieties.

Description

technical field [0001] The invention relates to the technical field of crop genetics and breeding, in particular to a wheat anti-ear germination gene TaZFP18 and its application. Background technique [0002] Pre-harvest sprouting (PHS) refers to the phenomenon that the crops encounter rainy weather before harvesting, which leads to the direct germination of seeds on the ears. Ear germination before harvest in wheat will seriously reduce the stability of yield and quality. The longer the time of continuous rainy weather before harvest, the more serious the ear germination hazard. Therefore, cultivating wheat varieties with strong ear germination resistance can help reduce this risk. [0003] The factors affecting ear germination mainly include external environmental factors and internal factors. Light and temperature are the main environmental factors; internal factors mainly include seed dormancy, seed coat color, panicle shape, grain water absorption characteristics, α-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12Q1/6895
CPCC07K14/415C12Q1/6806C12Q1/6895C12Q2531/113
Inventor 王升星朱玉磊张海萍常成姜昊吴曾云曹佳佳卢杰司红起马传喜
Owner ANHUI AGRICULTURAL UNIVERSITY
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