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Method for extracting and separating anthocyanin from red cabbage

A technology of purple cabbage and anthocyanin, which is applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of insignificant improvement in product purity and many impurities adsorbed.

Active Publication Date: 2016-04-20
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, macroporous adsorption resins are commonly used in China to separate and purify purple cabbage anthocyanins. The advantages of macroporous adsorption resins are that they have large adsorption capacity, can be used repeatedly, and can save costs at a certain cost; the disadvantage is that there are more impurities adsorbed. Purified product purity improvement is not very significant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Wash fresh purple cabbage, cut into about 1cm 2 Throw large or small lumps into the extraction tank, then add ethyl acetate for extraction, the extraction material-liquid ratio is 1:8 (w / v), the extraction temperature is 45°C, the extraction time is 80min, and the ethyl acetate solvent is collected. The ethyl acetate solvent was washed with deionized water, and the amount (volume) of deionized water was 7 times that of ethyl acetate (volume). The ethyl acetate solvent washed with water was concentrated under reduced pressure, the vacuum degree was -0.08Mpa, the concentration temperature was 50°C, the solvent was recovered, and the extract was obtained. The water content in the extract was 4.15%. Dissolve the obtained extract with 8 times the amount of methanol, and the dissolution temperature is 45° C., then centrifuge to collect the centrifugate. Adopt simulated moving bed chromatographic separation technology to separate anthocyanins from the centrifuged liquid. The ...

Embodiment 2

[0022] Wash fresh purple cabbage, cut into about 1cm 2 Throw large or small lumps into the extraction tank, then add ethyl acetate for extraction, the extraction solid-liquid ratio is 1:10 (w / v), the extraction temperature is 50°C, the extraction time is 90min, and the ethyl acetate solvent is collected. The ethyl acetate solvent was washed with deionized water, and the amount (volume) of deionized water was 6 times that of ethyl acetate (volume). The ethyl acetate solvent washed with water was concentrated under reduced pressure, the vacuum degree was -0.06Mpa, the concentration temperature was 55°C, the solvent was recovered, and the extract was obtained. The water content in the extract was 3.17%. The obtained extract was dissolved with 5 times the amount of methanol, and the dissolution temperature was 50° C., then centrifuged, and the centrifuged liquid was collected. Adopt simulated moving bed chromatographic separation technology to separate anthocyanins from the centr...

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PUM

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Abstract

The invention belongs to the field of natural organic chemistry and relates to a method for extracting and separating anthocyanin from red cabbage. The method comprises the following step: firstly, slicing red cabbage; utilizing a solvent (ethyl acetate) for extraction; using deionized water to wash ethyl acetate to remove water-soluble components; concentrating the ethyl acetate solution to obtain an extract; dissolving and cleaning the extract with methanol; using the simulated moving bed chromatographic separation technology for separation to obtain the anthocyanin product. The method has the advantage that as the simulated moving bed chromatographic separation technology is adopted for separation of anthocyanin from red cabbage, the obtained red cabbage anthocyanin product is high in purity. The prepared red cabbage anthocyanin can be widely applied to the fields of health food, cosmetics and the like.

Description

technical field [0001] The invention belongs to the field of natural organic chemistry and relates to a method for preparing purple cabbage anthocyanins from purple cabbage. Background technique [0002] Anthocyanin is a water-soluble pigment that can change color with the acidity and alkalinity of the cell fluid. The cell fluid is acidic and reddish, and the cytosol is alkaline and blue. Anthocyanin is one of the main pigments that make up the color of petals and fruits. Anthocyanins are secondary metabolites of plants, which play an important role in physiology. Anthocyanins are pure natural anti-aging nutritional supplements. Studies have proved that they are the most effective plant antioxidants found by humans today. Its antioxidant properties Fifty times higher than vitamin E and twenty times higher than vitamin C. Its bioavailability to the human body is 100%, and it can be detected in the blood 20 minutes after taking it. It has a significant effect on enhancing vi...

Claims

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Application Information

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IPC IPC(8): C07H17/065C07H1/08C07D311/62C09B61/00
CPCC07D311/62C07H1/08C07H17/065C09B61/00
Inventor 姚德坤苏学辉万莉
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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