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Monoclonal antibody Q206 and application

An antibody and sequence listing technology, applied in applications, antibodies, antibody medical components, etc., to achieve far-reaching social significance and significant application value.

Active Publication Date: 2016-05-04
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no similar emergency medicine in our country. Once a Chinese citizen is infected with Ebola virus, the consequences will be disastrous

Method used

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  • Monoclonal antibody Q206 and application
  • Monoclonal antibody Q206 and application
  • Monoclonal antibody Q206 and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, the discovery of antibody

[0034] 1. Preparation of GPΔm protein

[0035] 1. Construction of recombinant plasmids

[0036] (1) Synthesize the double-stranded DNA molecule shown in Sequence 2 of the Sequence Listing.

[0037] The double-stranded DNA molecule shown in sequence 2 of the sequence listing encodes the protein shown in sequence 1 of the sequence listing, wherein the open reading frame is nucleotides 18-1451 from the 5' end of sequence 2. In sequence 1 of the sequence listing, amino acid residues 1 to 19 from the N-terminal form the signal peptide, and amino acid residues 472 to 477 form the His 6 Label. The expected molecular weight of the protein shown in Sequence 1 of the Sequence Listing is 200 kDa.

[0038] (2) Double-digest the double-stranded DNA molecule obtained in step 1 with restriction endonucleases BamHI and NotI, and recover the digested product.

[0039] (3) The plasmid pcDNA3.1(+) was double-digested with restriction enzymes ...

Embodiment 2

[0065] Embodiment 2, the preparation of antibody

[0066] 1. Construction of recombinant plasmids

[0067] The double-stranded DNA molecule shown in sequence 4 of the sequence listing is inserted into the PMD18T vector to obtain a heavy chain expression vector.

[0068] The double-stranded DNA molecule shown in sequence 6 of the sequence listing is inserted into the PMD18T vector to obtain a light chain expression vector.

[0069] 2. Construction of recombinant cells

[0070] The heavy chain expression vector and the light chain expression vector were co-transfected into 293T cells to obtain recombinant cells.

[0071] 3. Antibody preparation

[0072] 1. Take the recombinant cells obtained in step 2, culture them in DMEM medium containing 2% fetal bovine serum for 72 hours, then centrifuge at 4000 rpm for 30 minutes at 4° C., and collect the supernatant.

[0073] 2. Affinity chromatography

[0074] Column specifications for affinity chromatography: length 3cm, inner diame...

Embodiment 3

[0080] Embodiment 3, the effect of antibody

[0081] One, the preparation of EBOV pseudovirus

[0082] 1. Insert the double-stranded DNA molecule shown in sequence 8 of the artificially synthesized sequence listing between the BamHI and NotI restriction sites of the plasmid pcDNA3.1(+) to obtain the recombinant plasmid pcDNA3.1-GPΔmuc. The double-stranded DNA molecule shown in sequence 8 of the sequence listing encodes the protein shown in sequence 7 of the sequence listing (GPΔmuc protein of EBOV).

[0083] 2. The recombinant plasmid pcDNA3.1-GPΔmuc and the plasmid pNL4-3R-E-luciferase were co-transfected into 293T cells to obtain recombinant cells.

[0084] The plasmid pNL4-3R-E-luciferase contains all the genes of the HIV-1 virus genome (the difference from the wild HIV-1 virus genome is only that the envelope gene has a frameshift). Recombinant plasmid pcDNA3.1-GP△muc and plasmid pNL4-3R-E-luciferase were co-transfected into host cells to express EBOV pseudovirus. Only ...

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Abstract

The invention relates to a monoclonal antibody Q206 and application. The protected monoclonal antibody Q206 is an IgG antibody, and consists of a light chain and a heavy chain, wherein a CDR1 (complementarity determining region 1), a CDR2 and a CDR3 in a variable area of the heavy chain are sequentially 45th to 52nd amino acid residues, 70th to 79th amino acid residues and 118th to 128th amino acid residues from an N-terminal in a No.3 sequence of a sequence table; a CDR1, a CDR2 and a CDR3 in a variable area of the light chain are sequentially 45th to 53rd amino acid residues, 77th to 73rd amino acid residues and 110th to 123rd amino acid residues from an N-terminal in a No.5 sequence of the sequence table. The monoclonal antibody Q206 has the advantage that the important application value on the prevention and control of a Zaire subtype of an Ebola virus is realized.

Description

technical field [0001] The invention relates to a monoclonal antibody Q206 and its application. Background technique [0002] Ebola virus is one of the deadliest infectious viruses known to mankind so far, with an average case fatality rate of about 50%. Due to the high fatality rate of Ebola hemorrhagic fever, and currently there is no prevention or treatment available for widespread clinical use. The Ebola virus is listed as a Biosafety Level 4 virus and is considered one of the tools of bioterrorism. [0003] The Ebola outbreak that began in West African countries in the spring of 2014 has aroused widespread concern around the world. As of February 27, 2015, the number of confirmed and suspected cases of Ebola virus has reached 23,825, and the death toll has reached 9,660. The United States and some European countries have already had imported transmission and death cases. Therefore, the prevention and control of Ebola virus is the top priority of all countries in the ...

Claims

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Application Information

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IPC IPC(8): C07K16/10C12N15/13A61K39/42A61P31/14
CPCA61K39/00C07K16/10C07K2317/565
Inventor 张林琦张绮史宣玲王若珂左亚男
Owner TSINGHUA UNIV
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