Magnetic bead based nucleic acid extraction device and method

Abstract

The invention provides a magnetic bead based nucleic acid extraction device. The magnetic bead based nucleic acid extraction device comprises a deep-well multi-well plate, a magnetic adsorption mechanism, a position moving mechanism and an oscillation mechanism, wherein the magnetic adsorption mechanism comprises a magnetic bar fixing base and a magnetic bar movably arranged on the magnetic bar fixing base; the position moving mechanism comprises a ball screw and a driving motor, and two ends of the ball screw are connected with the magnetic bar fixing base and the driving motor respectively; the oscillation mechanism comprises a carrier and a main oscillation unit connected with the carrier; the magnetic bar fixing base, the ball screw and the driving motor are all located in the carrier; the deep-well multi-well plate is detachably arranged on the upper surface of the carrier. According to the magnetic bead based nucleic acid extraction device, layer-by-layer solution treatment can be realized through the position moving mechanism, magnetic separation time is shortened, magnetic adsorption is thorough, and magnetic beads are not carried away; meanwhile, solution transfer, magnetic adsorption and oscillation can be performed in the same position, so that transfer between deep-well multi-well plates through a manipulator is avoided, and magnetic adsorption time and cost are reduced.

Description

technical field [0001] The invention relates to the technical field of nucleic acid extraction devices, and more specifically, to a magnetic bead nucleic acid extraction device and method. Background technique [0002] With the popularization of genetic testing, personalized drug delivery, prenatal diagnosis, etc., in today's pursuit of high-throughput and automation in various fields of the biological industry, the limitations of traditional DNA extraction methods are becoming more and more obvious. Since the extraction of nucleic acid by the magnetic bead method can realize automatic extraction and large-scale operation, and the operation is simple and the time is short, the extraction of nucleic acid by the magnetic bead method has attracted more and more attention. [0003] Generally, the extraction steps of nucleic acid extraction by the magnetic bead method mainly include: (1) lysis; (2) binding; (3) washing; (4) separation. The specific description is that cells or t...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a magnetic bead method nucleic acid extraction device and method to solve the problem that the nucleic acid extraction device described in the background technology needs to be moved row by row to complete the magnetic adsorption, so that the time coordination is not strong

Images