Check patentability & draft patents in minutes with Patsnap Eureka AI!

Manufacturing method of culture dish for culturing stem cell aggregate

A production method and a petri dish technology are applied in the field of bio-regenerative medicine to achieve the effects of convenient operation, strong survival ability and high directional differentiation ability

Inactive Publication Date: 2016-05-04
SHANGHAI UNIV
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the technical problem of culturing stem cell aggregates in the prior art, the object of the present invention is to overcome the deficiencies in the prior art and provide a method for making a culture dish for cultivating stem cell aggregates, which can be directly used for culturing stem cell aggregates body

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Manufacturing method of culture dish for culturing stem cell aggregate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] In this embodiment, a method for making a petri dish for cultivating stem cell aggregates is specifically: under stirring at room temperature, take 0.06 g of chitosan powder with a deacetylation degree of 80% and disperse it in 6 ml. In the deionized water, acetic acid was added dropwise to adjust the pH to 4-5, so that it was completely dissolved, and the concentration of the chitosan solution was 1 wt%. A polypropylene petri dish with a diameter of 120mm was selected, and the 6ml chitosan solution with a deacetylation degree of 80% was evenly coated on a traditional petri dish, and after drying at room temperature for 48 hours at 25°C, the prepared coated The petri dish covered with the film material was sequentially soaked in gradient absolute ethanol solution to remove residual acetic acid, and then dried again at room temperature for 48 hours to prepare a petri dish that can be used for culturing stem cell aggregates.

[0021] In this embodiment, a method for culti...

Embodiment 2

[0023] This embodiment is basically the same as Embodiment 1, especially in that:

[0024] In this embodiment, a method for making a petri dish for cultivating stem cell aggregates is as follows: under stirring at room temperature, take 0.06 g of homemade chitosan powder with a degree of deacetylation of 70% and disperse it in 6 ml. In the deionized water, acetic acid was added dropwise to adjust the pH to 4-5, so that it was completely dissolved, and the concentration of the chitosan solution was 1 wt%. A polypropylene petri dish with a diameter of 120mm was selected, and the 6ml chitosan solution with a deacetylation degree of 70% was evenly coated on a traditional petri dish, and after drying for 48 hours at room temperature at 25°C, the prepared coated The petri dish covered with the film material was sequentially soaked in gradient absolute ethanol solution to remove residual acetic acid, and then dried at room temperature for 48 hours to prepare a petri dish for culturin...

Embodiment 3

[0027] This embodiment is basically the same as the previous embodiment, and the special features are:

[0028] In this embodiment, a method for making a petri dish for cultivating stem cell aggregates is specifically: under stirring at room temperature, add 3 mol / L NaOH dropwise to dissolve a certain amount of poly-L-glutamic acid in deionized water, and adjust the pH of the poly-L-glutamic acid solution to neutral, and then sequentially add quantitative NHS and EDC powder to the poly-L-glutamic acid solution to activate the side chain carboxyl group on the poly-L-glutamic acid to control the poly-L-glutamic acid - The molar ratio of glutamic acid, NHS and EDC is 1:1:5. After activating the side chain carboxyl group of poly-L-glutamic acid for 8 hours, add a certain amount of dissolved self-made deacetylation degree to the mixed system with a deacetylation degree of 60 % pH is the chitosan solution of 4-5, carries out chemical cross-linking, and the carboxyl content on the co...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a manufacturing method of a culture dish for culturing stem cell aggregate. According to the anti-cell adhesion principle, a hydrophilic material single-component or multicomponent crosslinking system is coated onto a common culture dish surface in different coating modes, and different drying processes are performed to obtain the culture dish for culturing stem cell aggregate. The culture dish can stop the adherent adhesion of cells, so that the stem cells spontaneously aggregate to form the stem cell aggregate; the formed stem cell aggregate contains abundant cell-cell interactions, simulates the microenvironment of the in-vivo cells, and has excellent dryness maintenance capacity and induced differentiation capacity. The culture dish prepared by the method can be directly used for culturing the stem cell aggregate, is convenient to operate, and is efficient and controllable, so that the stem cell aggregate can be more widely used for cytological research, stem cell therapy or tissue engineering.

Description

technical field [0001] The invention belongs to the technical field of bioregenerative medicine, and in particular relates to a method for cultivating stem cell aggregates and a method for making a culture dish for cultivating stem cell aggregates. Background technique [0002] Stem cells are a type of pluripotent cells with self-replicating ability, which can be induced to differentiate into bone, cartilage, nerve, fat and other cells. They can be used as seed cells for tissue engineering and have received extensive attention since their discovery. [0003] The traditional culture method of stem cells is generally a two-dimensional monolayer adherent culture on a petri dish. At present, studies have found that compared with the traditional two-dimensional monolayer adherent culture of stem cells, stem cells cultured in three-dimensional aggregates of stem cells have the following advantages: there are extensive cell-cell interactions in stem cell aggregates, which better si...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/0775
CPCC12N5/0667C12N2533/30C12N2533/70C12N2533/72C12N2533/74C12N2533/80
Inventor 尹静波宋丽张坤玺张丽丽
Owner SHANGHAI UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com