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Fusarium moniliforme qualitative and standard sample in bean flour and preparation method thereof

A technology of Fusarium moniliforme and standard samples, which is applied in the biological field and can solve the problems of no standardization, traceability awareness and understanding confusion, etc.

Active Publication Date: 2016-05-04
DALIAN OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The status of traceability description of classification and grading national standard samples shows that people's awareness and understanding of the traceability of such standard samples is in a relatively chaotic stage, and there is no unified specification

Method used

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  • Fusarium moniliforme qualitative and standard sample in bean flour and preparation method thereof
  • Fusarium moniliforme qualitative and standard sample in bean flour and preparation method thereof
  • Fusarium moniliforme qualitative and standard sample in bean flour and preparation method thereof

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Embodiment

[0031] Using the classification and grading theory of standard substances, establish a reference system and reference indicators for the determination and traceability of the physical qualitative standard samples of Fusarium moniliforme in soybean flour, including the following steps:

[0032] The first step: the establishment of the reference system for the determination and traceability of reference materials:

[0033] (1) Taxonomic status of Fusarium moniliforme

[0034] The taxon characteristic value of a fusarium in the polluted soybean flour sample is Fusarium moniliforme (species), wherein the characteristic used for classification is the taxon status of the fusarium, and the characteristic value of the fusarium (measured object) For Fusarium moniliforme (species). The taxonomic status characteristic value set of Fusarium (genus) includes Orthospora group, Lyser group, Paleomyces group, Cladosporium group, Brick red group, Meili group, Matt group, spider silk group, Ep...

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Abstract

The invention belongs to the technical field of biology and particularly relates to a fusarium moniliforme qualitative and standard sample in bean flour and a preparation method thereof. According to the technological index that fusarium moniliforme has taxonomic significance, the problems of a fusarium moniliforme material standard sample tracing reference system and reference indexes are solved, the technological index that fusarium moniliforme has taxonomic significance is applied to definite value analysis of the standard sample, toxin-producing fungus fusarium moniliforme definite in source is used for simulating naturally-polluted bean flour samples in a national biosafety standard sample system framework on the basis of a research result of a qualitative testing theory of the standard sample, fusarium moniliforme ACCC36127 spore polluted sterile bean flour in the logarithmic phase is acquired and is flapped and mixed through a mixing instrument, sub-packaging is performed, freeze-drying is performed, and the fusarium moniliforme material standard sample complete in biology and toxin-producing information is prepared, the fusarium moniliforme material standard sample which has the real tracing significance is acquired and belongs to domestic first-time development of classifying and grading standard samples, and great significance is achieved.

Description

technical field [0001] The invention belongs to the field of biological technology, in particular to a qualitative standard sample of Fusarium moniliforme in soybean flour and a preparation method thereof. Background technique [0002] The World Food and Agriculture Organization (FAO) reports that about 25% of the world's crops are polluted by fungi and their mycotoxins every year, and about 2% of the crops lose their nutritional value and economic value due to serious pollution. Direct and indirect losses caused by pollution may reach tens of billions of dollars. [0003] Among the currently known mycotoxins, Fusarium moniliforme is an important plant pathogen that harms crops such as wheat, corn, rice, and vegetables, causing severe yield reduction. The mycotoxin zearalenone produced has Strong reproductive toxicity and Qi effect. At present, for the detection of mycotoxins, our country mainly uses physical and chemical methods, which can only detect the toxins that have...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 蒋丹刘淑艳万超丁健白璐郑江
Owner DALIAN OCEAN UNIV