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Application of geranium water extract in the preparation of anti-angiogenesis drugs

An anti-angiogenesis and geranium technology, which can be used in anti-tumor drugs, drug combinations, cardiovascular system diseases, etc., can solve the problems of easy drug resistance, expensive drugs, and single drug target.

Active Publication Date: 2020-02-11
上海砥石生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But these drugs are very expensive
[0005] At present, there is only one real anti-tumor angiogenesis drug approved by CFDA in China (Enstar, Simcere Pharmaceuticals, launched in 2005), but because Endostar (recombinant human endostatin injection) is a macromolecular protein drug , the production of such high-purity and safe preparations requires a high technical threshold, and this type of drug has a short half-life in the body, a single drug target, easy to develop drug resistance, and is expensive, which limits the use of these active protein drugs. clinical application

Method used

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  • Application of geranium water extract in the preparation of anti-angiogenesis drugs
  • Application of geranium water extract in the preparation of anti-angiogenesis drugs
  • Application of geranium water extract in the preparation of anti-angiogenesis drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] The preparation of embodiment 1 Geranium water extract

[0083] (1) Take 50 g of geranium herb powder, add 400 ml of deionized water, extract under reflux for 1 hour, and filter to obtain the filtrate.

[0084] (2) Add 300 ml of deionized water to the filter residue after the first step of filtration, reflux for extraction for 1 hour, and filter to obtain a filtrate.

[0085] (3) The two filtrates were combined, and recovered to dryness at 70°C to obtain the aqueous extract of Geranium, which was frozen and stored at -20°C for future use.

Embodiment 2

[0086] The preparation of embodiment 2 geranium ethanol extract

[0087] (1) Take 100 g of geranium herb powder, add 800 ml of absolute ethanol, reflux for extraction for 1 hour, and filter to obtain the filtrate.

[0088] (2) Add 600 ml of absolute ethanol to the filter residue after the first step of filtration, reflux for extraction for 1 hour, and filter to obtain the filtrate.

[0089] (3) The two filtrates were combined and recovered to dryness at 60°C to obtain the ethanol extract of Geranium, which was frozen and stored at -20°C for future use.

Embodiment 3

[0090] Example 3 Evaluation of the inhibitory effect of geranium water extract and alcohol extract on zebrafish intersegmental blood vessel (ISV) generation model

[0091] The purpose of the present invention is to evaluate the anti-angiogenic efficacy of geranium water extract by using zebrafish body intersegmental vessel (ISV) generation model. This in vivo model can be used for pharmacodynamic evaluation of anti-angiogenic drugs. Intersegmental angiogenesis in zebrafish is a complete dynamic process. The intersegmental vascular endothelial cells sprout (sprouts) from 20hpf, and form the main intersegmental vascular network at about 30-31hpf, such as the dorsal long axis vessel (DLAV) And intersegmental vessels (ISVs), 48hpf basically formed a complete axial vascular network, and the complete intersegmental vessels (ISVs) were clearly visible at this time.

[0092] The intact intersegmental vessels mainly refer to the vessels connecting the dorsal aorta (DA) and the dorsal ...

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Abstract

The invention provides an application of a Geranium wilfordii Maxim. aqueous extract in the preparation of anti-angiogenesis drugs. The anti-angiogenesis effect of the Geranium wilfordii Maxim. aqueous extract is found in the invention for the first time. In vivo pharmacodynamic experiments using a zebra fish angiogenesis model confirm that the Geranium wilfordii Maxim. aqueous extract can substantially inhibit angiogenesis of zebra fish, so the Geranium wilfordii Maxim. aqueous extract has anti-angiogenesis activity, can be used as an angiogenesis inhibitor, and can be applied in treatment of tumors, arthritis, skin and ophthalmologic diseases, atherosclerosis, vascular dementia, endometriosis and other angiogenesis-dependent and angiogenesis-related diseases.

Description

technical field [0001] The invention relates to the application of Chinese herbal medicine extracts, in particular to the application of a geranium water extract in the preparation of anti-angiogenesis drugs. Background technique [0002] Angiogenesis is the process of sprouting new capillary-based vascular systems on existing vascular beds. At the beginning of angiogenesis, vascular endothelial cells dedifferentiate first. Under various conditions, the vascular basement membrane becomes thinner or disappears, endothelial cells activate, proliferate, migrate, and a new basement membrane forms to cover endothelial cells and vascular smooth muscle cells, and eventually form new blood vessels. [0003] Angiogenesis is highly related to many major human diseases, such as malignant tumors, atherosclerosis, arthritis, skin and eye diseases (age-related macular degeneration, diabetic retinal degeneration), vascular dementia, endometriosis, etc. [0004] Malignant tumor is one of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P35/00A61P17/06A61P9/00A61P19/02A61P27/02A61P9/10A61P15/00A61P25/28
Inventor 费俭吴转斌孙瑞林王红兵王铸钢
Owner 上海砥石生物科技有限公司