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Mercapto-peptide, preparation method and applications thereof

A technology of thiol peptides and thiol groups, which is applied in the field of animal protein waste treatment and resource utilization, can solve the problems of low passivation efficiency of organic passivators, affecting plant nutrient absorption, easy to change soil characteristics, etc., and achieves aggregate structure The effect of loosening and uniformity, promoting plant growth and strengthening mutual relationship

Pending Publication Date: 2016-05-11
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the current advantages and disadvantages of soil in-situ passivators at home and abroad, that is, inorganic passivators can easily change soil characteristics, affect the absorption of plant nutrients, and easily cause secondary pollution, while organic passivators have low passivation efficiency and high speed. Slow, very easy to cause non-point source pollution, seriously affecting the surrounding water sources and other issues, the purpose of the present invention is to provide a thiol peptide and its preparation method and its application as an in-situ passivator in the treatment of heavy metal-contaminated soil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] In this embodiment, animal feathers are used as raw materials to prepare thiol peptides, including the following steps:

[0032] Feathers with a protein content of 80-85% are directly (or crushed) put into an autoclave, then water equivalent to 400-700% of the feather weight (W / W) is added to the autoclave, and then Add potassium hydroxide equivalent to 2-5% of the weight of feathers into the reaction kettle, heat the material to 121°C-135°C with steam, and keep it warm for 1-5 hours. If the feathers have all become peptone-like or completely dissolved After the hydrolysis is completed, the hydrolyzate is concentrated to a concentration of 12-23 Baume to obtain the water-soluble thiol peptide.

[0033] More specifically, at 10m 3 Add 800kg of feathers, 40kg of potassium hydroxide, and 3600kg of water into the reaction kettle, heat it to 130°C with 0.2Mpa to 0.85Mpa of steam, keep it warm for 1.2 to 3 hours, and observe by sampling. If all the feathers have turned into ...

Embodiment 2

[0035] In this example, waste animal blood was used as raw material to prepare thiol peptides, including the following steps:

[0036] at 10m 3 Add 4000kg of animal blood (80% water content, 800kg dry matter weight, and 85% protein content in dry matter) to the batching bucket, add 40kg potassium hydroxide, and add 5000kg water (based on the water content of 800kg dry matter) , put in 0.2-0.7Mpa steam and heat to 120-145°C, keep it warm for 1.5-3.5h until it is completely dissolved, then concentrate the hydrolyzed solution in a multi-effect evaporator until it reaches 15-18 Baume, and measure its pH value with pH test paper 8.8-11.6, this concentrated solution is the multi-functional water-soluble thiol peptide.

Embodiment 3

[0038] In this embodiment, animal feathers are used as raw materials to prepare thiol peptides, including the following steps:

[0039] Feathers with a protein content of 80-85% are directly (or crushed) put into an autoclave, then water equivalent to 700-900% of the weight of the feathers (W / W) is added to the autoclave, and then Add potassium hydroxide equivalent to 1-2% of the weight of feathers into the reaction kettle, feed steam to heat the material to 80°C-120°C, and keep it warm for 35-40 hours. When sampling or opening the exhaust valve, hydrogen sulfide gas will be produced. When the temperature is lowered to 90-105°C, if the feathers have all become peptone-like or completely dissolved, the hydrolysis can be completed, and the hydrolyzed solution is concentrated to a concentration of 12-23 Baume, that is, the sulfhydryl group-containing High molecular weight thiol peptides. The molecular weight of the mercapto peptide determined by high performance liquid chromatog...

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Abstract

The invention discloses a mercapto-peptide, a preparation method and applications thereof, and belongs to the technical field of protein waste treatment and utilization. The mercapto-peptide is water soluble, the average molecular weight is 3000 to 20000 daltons, and nitrogen accounts for 12.0 to 14.0 wt% of mercapto-peptide. According to the preparation method, a proper amount of potassium hydroxide and water is added into protein wastes especially protein containing animal leftovers, which contain a large amount of disulfide bonds, such as feathers, quills, hoofs, horns, hairs, blood, and the like; and then reactions are carried out at a constant temperature of 70 to 186 DEG C for 0.5 to 40 hours so as to obtain the mercapto-peptide. The produced water-soluble mercapto-peptide can be used as an in-situ passivating agent to passivate heavy metal ions (cadmium, lead, copper, zinc, arsenic, alum, mercury, etc.) in soil, the effective state concentration of heavy metal ions in soil can be reduced, and thus the heavy metal content of planted crops becomes qualified. Furthermore, the mercapto-peptide can be used as a base fertilizer and topdressing, and is capable of promoting the degradation of organic substances in soil, improving the fertilizing effect, and promoting the growth of plants.

Description

technical field [0001] The invention belongs to the technical field of protein-containing waste, especially the treatment and resource utilization of animal protein-containing waste. Specifically, the invention relates to a thiol peptide and its preparation method and as an in-situ passivator in the treatment of heavy metal pollution. Soil applications. Background technique [0002] my country's arable land soil pollutants exceeded the rate of 19.4%, cadmium, nickel, copper, arsenic, mercury, lead, DDT (di-p-chlorophenyl trichloroethane) and polycyclic aromatic hydrocarbons became the chief culprits. The total point exceeding standard rate of the national soil is 16.1%, of which the proportions of slightly, lightly, moderately and heavily polluted points are 11.2%, 2.3%, 1.5% and 1.1% respectively. From the perspective of pollutants exceeding the standard, the 8 kinds of inorganic pollutants such as cadmium, mercury, arsenic, copper, lead, chromium, zinc and nickel exceeded...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/12C05G3/00C05G3/04C05F1/00C05G3/80
CPCC05F1/005C05G3/00C05G3/80C07K1/12Y02A40/20
Inventor 李亚东王行国李龙盘陈红兵李顺意杨兰芳李洋李海波倪红高琪李思彤
Owner HUBEI UNIV
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