Anti-foot-and-mouth disease vaccine composition and preparation method and application thereof

A vaccine composition and composition technology, applied in the direction of biochemical equipment and methods, methods based on microorganisms, medical preparations containing active ingredients, etc., can solve the problems of short validity period, incompletely killed or fully attenuated viruses, and pollution And other issues

Active Publication Date: 2016-05-11
PU LIKE BIO ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also the following disadvantages: 1. The virus must be produced in highly sealed equipment to prevent contamination of the surrounding environment; during the production process, the virus may not be completely killed or fully attenuated, which will cause the vaccine to contain highly toxic pathogens. The disease substance, which in turn causes the disease of the immunized animals and causes the disease to spread on a large scale
2. The validity period is very short, and often need

Method used

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  • Anti-foot-and-mouth disease vaccine composition and preparation method and application thereof
  • Anti-foot-and-mouth disease vaccine composition and preparation method and application thereof
  • Anti-foot-and-mouth disease vaccine composition and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Protein expression of foot-and-mouth disease having sequences SEQ ID NO.1, 3, 5, 7, 9, 11, 13, 15, 17

[0051] 1. Preparation of foot-and-mouth disease gene fragments used as templates

[0052] The O-type foot-and-mouth disease VP0, VP3, VP1 gene shown in the sequence SEQIDNO.1, 3, 5, the Asian type 1 foot-and-mouth disease VP0, VP3, VP1 gene shown in the sequence SEQIDNO. The full-length genes of type A FMD VP0, VP3, and VP1 shown in 15 and 17 were synthesized by Sangon Bioengineering (Shanghai) Co., Ltd. The full lengths of the synthesized gene fragments are 909bp, 660bp, 639bp, 909bp, 657bp, 627bp, 909bp, 663bp, 636bp, respectively. The foot-and-mouth disease gene template of the present invention is prepared on the basis of the artificially synthesized foot-and-mouth disease gene fragment.

[0053] 2. Construction of foot-and-mouth disease gene expression vector

[0054] FMD gene template synthesized in the previous step. Primers were designed respectively (see ...

Embodiment 2

[0068] Preparation of vaccine composition against foot-and-mouth disease

[0069] Get the O-type foot-and-mouth disease virus-like particles prepared in Example 1, the Asian type I foot-and-mouth disease virus-like particles and the A-type foot-and-mouth disease virus-like particles, and slowly add them to the adjuvant, and the process of adding is constantly stirring with a rotating speed of 800rpm emulsifier for 12min, mixing Evenly and stored at 4°C, it becomes the vaccine composition against foot-and-mouth disease. See Table 3 for specific ratios. The adjuvants suitable for the present invention may be adjuvants known to those skilled in the art. In this example, the adjuvant ISA206 (Seppic, France) was selected.

[0070] The composition ratio of the vaccine composition against foot-and-mouth disease of table 3

[0071]

Embodiment 3

[0073] Immunogenicity test of vaccine composition against foot-and-mouth disease

[0074] 1. Immunization procedure

[0075] 30 6-month-old healthy cattle that were negative for the above three antibodies were screened using the FMD O-type antibody ELISA detection kit, the Asian I-type ELISA antibody detection kit and the A-type antibody ELISA detection kit, and were randomly divided into 6 groups. 5 heads per group. Groups 1-5 are vaccine 1, vaccine 2, vaccine 3, vaccine 4, and vaccine 5 immunization groups prepared in Example 2 of the present invention, respectively, and group 6 is a PBS control group. The way of immunization in the immunization group was intramuscular injection of 1 ml in the neck, and the control group was immunized with the same amount of PBS. Blood was collected from each cow before vaccine immunization and every week after immunization until 21 days after immunization.

[0076] 2. Detection of antibody level

[0077] The collected sera were tested f...

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Abstract

The present invention relates to the field of veterinary biological products, and more specifically discloses a type-A foot-and-mouth disease virus-like particle and an anti-foot-and-mouth disease vaccine composition and a preparation method and application thereof in prevention and / or treatment of infections-related diseases caused by FMD (foot-and-mouth disease) virus. The vaccine composition comprises a type-O FMD virus-like particle and / or type-Asia-I FMD virus-like particle and / or the type-A FMD virus-like particle and an adjuvant.

Description

technical field [0001] The invention relates to the field of veterinary biological products. More specifically, the present invention relates to a vaccine composition against foot-and-mouth disease. The present invention also relates to the application of the vaccine composition for preventing and / or treating diseases related to infection caused by foot-and-mouth disease virus. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, highly contagious animal disease that can spread quickly and over long distances. It is the most contagious disease among mammals, and the infection of artiodactyls will cause significant economic losses worldwide. Animals affected by FMD include cattle, sheep, goats and pigs. The causative agent, foot-and-mouth disease virus (FMDV), is an aphthous sore virus of the picornavirus family. The virus is divided into seven serotypes (A, O, C, Asian, SAT1, SAT2, and SAT3), of which type A foot-and-mouth disease virus is more prevalen...

Claims

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Application Information

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IPC IPC(8): C07K7/04A61K39/135A61P31/14C12R1/93
Inventor 张许科袁于人孙进忠陈红英肖燕田克恭
Owner PU LIKE BIO ENG
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