Unlock instant, AI-driven research and patent intelligence for your innovation.

Fertility gene and application thereof

A fertility gene and fertility technology, which is applied to plant hybrid breeding, fertility gene FL2 and its mutants and its application in hybrid breeding, can solve the problem of high cost of seed production, reduction of hybrid seed purity, and seed production risk. increase, etc.

Inactive Publication Date: 2016-05-11
SHENZHEN INST OF MOLECULAR CROP DESIGN +1
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Three-line hybridization requires specific restorer lines and maintainer lines, which not only complicates the seed production process, but also greatly limits the utilization of heterosis for different varieties of resources
At present, most of the sterile lines used in the two-line hybridization are light-temperature-sensitive sterile lines, and their fertility is affected by changes in temperature and light. The instability of these environmental factors will affect the stability of the sterile lines, resulting in self-fertilization and reduced fertility. The purity of hybrid seeds is compromised, which increases the risk of seed production
Moreover, the available sterile lines for two-line hybridization using the current technology are very limited. For example, there are almost no good two-line hybrid combinations in japonica rice varieties, which limits the full utilization of variety resources.
[0006] In order to solve the defects in the current rice hybrid breeding methods, such as the stability of the sterile line, the limitation of hybrid variety resources, the complexity of seed production technology, the high cost of seed production and other technical bottlenecks, people are trying new hybrid breeding techniques, new The hybrid breeding technology will make full use of the male sterility gene controlled by the recessive nuclear gene, construct a sterile line with stable fertility and not affected by the environment, remove the constraints of environmental factors on hybrid breeding, and eliminate potential risks in production; at the same time, the The recessive sterility gene used should be applicable to the vast majority of varieties, so that the utilization of heterosis resources can be greatly improved, and the problem of resource utilization of heterosis can be solved; The male sterile line of the male sterile line has stable fertility, is not affected by environmental conditions, and can be restored by wild-type transgenes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fertility gene and application thereof
  • Fertility gene and application thereof
  • Fertility gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Embodiment 1, rice male sterile mutant (osfl2) screening

[0110] The mutant was obtained by mutagenizing the indica rice Huanghuazhan seeds (M0) with EMS, the concentration and time of EMS mutagenesis were 0.7%, 12 hours, and the mutant pool (M1) was obtained from the seed plants of the M0 generation after they were fruited. Plants from the seeds of the M1 generation were used for screening at the seed maturity stage to obtain sterile plants. Sterile plants were regenerated by cutting rice piles, and pollen development and staining reactions of regenerated plants were detected by I2-KI staining during the reproductive period. One of the mutants showed no pollen type sterility (nopollen), named osfl2.

Embodiment 2

[0111] Embodiment 2, rice male sterile mutant (osfl2) genetic analysis

[0112] The osfl2 mutant sterile plants were crossed with the wild type Huanghuazhan, and all 80 F1 plants were fertile. Then the F1 generation was self-crossed, and among the 300 F2 generation plants obtained, 78 were pollen-free sterile, 222 plants were completely fertile, and the segregation ratio of sterile and fertile plants was close to 1:3 , showing that the mutation is controlled by a recessive nuclear gene.

Embodiment 3

[0113] Embodiment 3, rice male sterile mutant (osfl2) fertility stability analysis

[0114] In order to determine whether the osfl2 mutation's physicality is affected by ambient light and temperature, the F2 generation plants produced by crossing the sterile plants with wild-type Huanghuazhan were planted in Shenzhen, Sanya, Hunan, and Beijing, and the fertility and phenotypic segregation ratio of the plants were further observed. In all regions, the sterile plants:fertile plants showed a segregation ratio of 1:3 (Table 1), and the sterile plants regenerated from cutting rice piles were still sterile, indicating that the mutant's sterile traits were not affected by environmental factors .

[0115] Table 1: Segregation ratio of F2 generation of rice male sterile mutant (OsFL2) hybrid

[0116]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a fertility gene and an application thereof, and provides a fertility gene FL2, a mutant of the fertility gene FL2 and the application of the fertility gene FL2 and the mutant in crossbreeding, which includes breeding of the sterile lines and preparation of hybrid seeds.

Description

[0001] This application is a divisional application of Chinese patent application 201380004377.8 "A Fertility Gene and Its Application" with the filing date of November 7, 2013. technical field [0002] The invention belongs to the field of biotechnology, and specifically relates to a plant hybrid breeding method, including sterile line propagation and hybrid seed preparation, and more specifically relates to a fertility gene FL2 and its mutant and its application in hybrid breeding. technical background [0003] Hybrid breeding is an effective way to improve crop production. Hybrids usually have obvious yield, resistance and adaptability advantages over conventional varieties. The breeding cycle of hybrids is usually shorter and quicker than conventional breeding. At present, hybrid breeding has become the main breeding method for many crops. [0004] The selection of crop male sterile lines is a key link in hybrid breeding. The male sterile line is a plant individual with...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H1/02A01H5/00
CPCA01H1/02C07K14/415C12N15/8289C12N15/113C12N15/8218C12N15/8287C12N15/8231C12N15/829A01H5/00C12N2310/11A01H1/023A01H1/045A01H6/4636C12N15/00C12N15/823
Inventor 唐晓艳陈竹锋谢刚王娜卢嘉威李早霞
Owner SHENZHEN INST OF MOLECULAR CROP DESIGN