Method for preparing low-molecular chondroitin sulfate through electron beam irradiation

Abstract

The invention discloses a new method for preparing low-molecular chondroitin sulfate. The new method includes the steps that chondroitin sulfate products with the common molecular weight serve as raw materials, are degraded through electron beam irradiation and are dissolved and subjected to ultrafiltration after degradation, and the products are dried to obtain the low-molecular chondroitin sulfate. In the technology process that the low-molecular chondroitin sulfate is prepared from coarse chondroitin-sulfate materials, as multiple links such as enzyme preparation adding and chemical agent adding are replaced with pure physical means such as irradiation degradation, the technology process is simplified, the purity and the quality consistency of the product are improved, the problem that the environment is polluted in the enzymatic degradation preparing process and the chemical degradation preparing process is solved, the average molecular weight of the product is smaller than 10,000 Da, and the obtained product has the important effect on keeping the integrity of tissues. At present, the chondroitin sulfate serves as medicine and a dietary supplement to be widely applied to osteoarticular diseases, cardiovascular and cerebrovascular diseases and ophthalmic diseases.

Description

technical field [0001] The method relates to a new method for preparing low-molecular chondroitin sulfate by electron beam irradiation. Background technique [0002] Chondroitin sulfate (CS) is a type of glycosaminoglycan covalently linked to proteins to form proteoglycans. It is widely distributed in the extracellular matrix and cell surface of animal tissues. It is usually extracted from animal cartilage tissue. The relative molecular weight (Mr) is generally above 20000Da, and when the (Mr) is reduced to 10000Da or lower, it is called low molecular weight chondroitin sulfate. Compared with ordinary chondroitin sulfate, low-molecular chondroitin sulfate is easier to be absorbed by oral administration, so its bioavailability is higher, and its health care and medical biological activity is more obvious. It is considered to be a new generation (CS) product with good application Development prospects, studies have found that low-molecular-weight chondroitin sulfate can affec...

AI Technical Summary

Problems solved by technology

[0006] The disadvantages of enzymatic hydrolysis include: it is difficult to control the degradation degree of molecular weight, the acquisition cost of various enzymes is high, and the functional activity of enzyme preparation products is unstable, which leads to the need for repeated adjustment of subsequent process conditions (temperature, pH value, etc.), which increases the difficulty of production. After the reaction, the enzyme needs to be inactivated and separated, and the physical and chemical properties of the product batches are unstable

[0007] The main disadvantages of the chemical degradation method are: the problem of chemical additives remaining in the product, and the problem of environmental pollution emitted by enterprises

[0008] These two methods are especially obvious when preparing (CS) with a molecular weight of less than 10000Da, including a larger amount of alcohol consumed

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