Animal jugular vein microdialysis method

Abstract

The invention discloses an animal jugular vein microdialysis method. The method comprises the steps that 1, an animal is anesthetized, hair on the neck is shaved, the neck is disinfected, and one side of the neck is vertically cut open; 2, fat and connective tissue around the jugular vein are subjected to blunt dissection; 3, under the skin of the neck and back on the surgical side, the subcutaneous bursa cavity is subjected to blunt dissection, and a channel is pierced out; 4, a syringe needle of a separate trocar is used for piercing the blood vessel at the end, away from the heart, of the jugular vein, a cannula is pushed into the jugular vein, the syringe needle is pulled out, a microdialysis probe is inserted in the jugular vein through the cannula and fixed, and the cannula is taken out; 5, a catheter of the microdialysis probe is led out of the body, and an injection pump is connected to the catheter for injection; 6, after injection is over, the incision is sutured. According to the method, microdialysis is performed through a jugular vein intubation method, the advantages of a small sampling quantity, little tissue damage and more truthful and reliable data are achieved, continuous sampling and real-time monitoring can be performed on a sober animal which moves freely, quantitative analysis can be carried out, and the method can be used as an effective means for drug research on in-vivo or in-vitro compounds.

Description

technical field [0001] The invention relates to the biological field, in particular to an animal jugular vein microdialysis method. Background technique [0002] Information such as the distribution and concentration of endogenous and exogenous compounds in the body is generally analyzed by collecting blood or tissue homogenate. With the deepening of research, this conventional method gradually shows many limitations. For example, for endogenous compounds, plasma or tissue homogenate can only provide basic information such as concentration, but the release mode and dynamic balance of the compound in the body cannot be reflected. [0003] Microdialysis (Microdialysis) technology is a new technology that combines perfusion sampling and dialysis technology and gradually improves dynamic micro-biological sampling from living organisms. It has continuous sampling, dynamic observation, quantitative analysis, small sampling volume, It has the advantages of light tissue damage, ca...

AI Technical Summary

Problems solved by technology

At present, microdialysis technology has become one of the important research tools of experimental neurophysiology and neurochemistry, which can provide the necessary information of transmitter release, uptake and metabolism, but its application in other tissues and systems has not been reported yet.

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