Aspergillus sydowii with broad-spectrum pesticide degradation characteristics

A technique of Aspergillus polypolyphagoides and pesticides, applied in the field of environmental biology, can solve problems such as soil repair defects, lack of tolerance, and compound pesticide pollution, and achieve high application value

Inactive Publication Date: 2016-05-25
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Compound pesticide pollution often exists in my country's current industrial sites, and the types of pesticides are diversified. However, most of the efficient pesticide-degrading microorganisms obtained in the current research are specific, that is, only one kind of pesticide or a type of pesticide (such as organophosphorus or chrysanthemum) Esters pesticides) degrade, and there are obvious defects in the soil remediation of industrial sites polluted by compound pesticides
At the same time, microorganisms that only degrade specific pesticides cannot achieve efficient degradation of pesticides due to lack of tolerance to other t

Method used

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  • Aspergillus sydowii with broad-spectrum pesticide degradation characteristics
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  • Aspergillus sydowii with broad-spectrum pesticide degradation characteristics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] [Example 1] Screening of broad-spectrum pesticide-degrading bacteria

[0019] The experimental soil and water samples were collected from the rhizosphere and non-rhizosphere soil samples around the new plant of Qinong Chemical Co., Ltd., Qizhou Town, Hubei Province, and the water samples were also collected from 5 drainage outlets of the plant. After the water samples were collected, they were immediately stored in the laboratory at -20°C to kill insects, plant tissues and some viruses in the soil. After 10 hours of treatment, it was thawed at 4°C, and the excess soil samples were stored in a 4°C refrigerator. Take 10g soil sample or 10mL water sample, add 100mL sterilized liquid enrichment medium (containing sterile glass beads) (enrichment medium composition: peptone 10g / L, NaCl1.0g / L, KH 2 PO 3 1.0g / L, pH=7), shaking at 150rpm for 7 days. After culturing for 1 week, take 10 mL of the culture solution in the enriched medium, and add 50 mg / L trichlorfon (TR), malath...

Embodiment 2

[0025] [Example 2] identification of bacterial strains

[0026]Inoculate the purified bacterial strain on solid PDA medium (ingredients: potato 200g / L, glucose 20g / L, agar 20g / L, pH=7) and cultivate for 5 days to observe the bacterial colony morphological characteristics of the bacterial strain on the solid plate, and then pick some Colonies were observed under an optical microscope to observe the microstructure of the strain, and the morphological characteristics of the strain were recorded: the hyphae were fine and net-like, with a diameter of about 0.15 μm; the spores were brown in color, round in shape, and about 0.10 μm in diameter; The stem is slender, uniform in diameter, with obvious branches, forked branches with acute angles, and the branches are asymmetrical.

[0027] The identification of physical and chemical properties of the strain mainly detects carbon source utilization and nitrogen source utilization physiological and biochemical tests. The results are recor...

Embodiment 3

[0032] [Example 3] Degradation rate analysis of bacterial strains to trichlorfon, 2,4-D and beta-cypermethrin

[0033] Use liquid MSM medium, and set the final concentration after adding three kinds of pesticides to 100mg / L. Before the measurement, inoculate the strain in PDA slant medium for cultivation, activate and cultivate at 28°C for 72 hours, then take 1mL sterile water, Add in the slope, scrape the spores and hyphae of the fungus on the slope into sterile water to form a spore suspension, with a volume ratio of 3% (initial inoculation spore content 10 9 individual / mL, biomass <0.1g) was added to the culture medium. All cultures were shaken at 28°C for 5 days based on a shaking table with a rotation speed of 150 rpm (the trichlorfon experimental group needed to be cultured for 14 days), and the dry weight of the PAF-2 strain in each group was determined using high performance liquid chromatography (HPLC). Degradation rates of strains to trichlorfon, 2,4-D and beta-cype...

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Abstract

The invention discloses a fungus strain PA F-2 with broad-spectrum pesticide degradation characteristics. The fungus strain is named as Aspergillus sydowii, and is preserved in China Center for Type Culture Collection in Wuhan university on 7th Sep. 2015, the address is Wuhan university, Wuhan, China, and the preservation number is CCTCC NO.M2015513. The Aspergillus sydowii strain PA F-2 is obtained via selection, is capable of degrading 13 pesticides, and possesses extremely high application value in comprehensive treatment of pesticide polluted water body and soil, and industrial site compound pesticide pollution.

Description

technical field [0001] The invention belongs to the technical field of environmental biology, and in particular relates to a fungus aspergillus polydoctoris PAF-2 with broad-spectrum pesticide degradation characteristics. Background technique [0002] The widespread use of pesticides, while bringing fast and efficient crop yields to agricultural production, has also caused serious pollution to water bodies, soil and the atmospheric environment, and has also increased product safety issues for agricultural products. At present, the commonly used pesticide remediation methods are kiln incineration, thermal desorption and chemical treatment, but there are problems such as high cost, difficult remediation, and easy to cause secondary pollution. The bioremediation method established by using organisms (mainly microorganisms) in water and soil that can use organic pesticides as energy substances and degrade them into non-toxic or low-toxic compounds is recognized as an efficient, ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A62D3/02B09C1/10C02F3/34C12R1/66A62D101/04
Inventor 陈兰洲田江余臣磊赵剑赵瑞雪汪静
Owner WUHAN UNIV
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