High flux detection method for tumor-targeted drugs related genes mutation, primers and reagent thereof

A tumor targeting and gene technology, applied in the field of tumor genes, which can solve the problems of low sensitivity and so on

Active Publication Date: 2016-06-01
绍兴积准生物科技有限公司
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Problems solved by technology

The traditional gene mutation detection method can only detect a few mutation sites at a time, and the sensitivity is low, a

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  • High flux detection method for tumor-targeted drugs related genes mutation, primers and reagent thereof
  • High flux detection method for tumor-targeted drugs related genes mutation, primers and reagent thereof

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Embodiment Construction

[0092] In the 21st century, cancer has surpassed coronary heart disease and stroke to become the number one cause of death. Currently, the treatments for malignant tumors include surgery, chemotherapy, radiotherapy, immunotherapy, and targeted drug therapy. Molecular targeted therapy targets key molecules and key sites in the pathogenesis of tumors, has strong selectivity and low toxicity and side effects, and is the development direction and new breakthrough point of tumor treatment. However, the mechanism of tumorigenesis is complex and there are many mutation sites. Screening for gene mutations associated with tumor-targeted drugs is a prerequisite for targeted drug therapy. The U.S. FDA's Tumor Targeted Drug Therapy Manual requires genetic testing for cancer mutations before using targeted gene drugs. Sanger sequencing and fluorescent quantitative PCR are currently the traditional methods used to detect gene mutations associated with tumor-targeted drugs. Sanger sequencin...

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Abstract

The invention relates to the field of tumor gene, and discloses a high-flux sequencing detection method for tumor-targeted drugs related genes mutation, a group of primer pairs and a reagent thereof. The method is used for detecting mutation of tumor-targeted drugs related genes in an acceptor, the related genes comprise BRAF, EGFR, KRAS, NRAS, TP53, FGFR3, KIT, PIK3CA, RET, PTEN, and CTNNB1. The method comprises the following steps: extraction of sample DNA, target gene enrichment, primer sequence removal, joint connection, PCR amplification, library homogenization, library detection and high flux sequencing. The disclosed primer pairs enable multiple PCR enrichment and amplification on the tumor-targeted drugs related genes, the method can be used for detecting 115 mutation sites in the above 11 genes, a detection result can be a medication guidance for the tumor-targeted drugs, and provides a basis for exploitation of novel cancer drugs.

Description

technical field [0001] The present application relates to the field of tumor genes, in particular to a detection method for genes related to tumor targeting drugs, a set of primer pairs, a reagent and a kit. Background technique [0002] Tumor is the biggest disease that threatens human health today. In China, tumor has surpassed coronary heart disease and stroke to become the number one cause of death. While traditional chemotherapy treatment kills tumor cells, it also causes great damage to the patient's body. With the development of molecular biology and cell biology, the understanding of the mechanism of tumor occurrence and development, molecular targeted drug therapy has become an effective measure for tumor treatment. Targeted drugs aim at tumor-specific sites, and by maintaining a high local concentration, they can increase the specific lethality against tumors, have little effect on normal tissue cells, and avoid the side effects of chemotherapy. [0003] There is...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 张煜
Owner 绍兴积准生物科技有限公司
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