Slide glass cover plate for slide glass sample treatment
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A technology for specimen processing and slides, which is used in laboratory containers, support of flat carriers, preparation of samples for testing, etc. and other problems, to achieve the effect of ensuring reliability and repeatability, saving space, and sufficient heating process
Inactive Publication Date: 2016-06-01
ASCEND BIOTECH CO LTD
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However, none of the currently commonly used containers or carriers for slide specimen processing has the ability to be heated or to withstand heat
In the middle of the slide specimen processing, the slide specimen needs to be transferred to a heatable container, and then transferred to the operating table at room temperature, which increases the complexity of the operation process and operational errors
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[0049] Example of implementation: the slide cover plate has the following dimensions: the length of the depth positioning surface 2 is 54mm, the distance between the inner sides of the two width positioning blocks 3 is 20mm, D1=0.2mm, D2=0.5mm, the length of the capillary plane is 40mm, The width is 20mm, and the angle between the groove opening surface and the depth positioning surface is 60 degrees. The length of the slide is 75mm, and the width is 25mm. The thickness of the sliced specimen is about 0.004mm. Experiments have shown that a capillary gap is formed between the capillary plane of the slide and the cover plate, which can form a capillary siphon effect, and the reagent fills the gap when adding a sample.
[0050] In the immunohistochemical staining, the slide assembly of the present invention is directly immersed in the boiling heat repair solution and partially inserted into the boiling heat repair solution at the bottom and heated for 40 minutes. The gaps are ...
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Abstract
The invention discloses a slide glass cover plate for slide glass sample treatment. The slide glass cover plate comprises a capillary plane, a depth locating face, a width locating block, a bottom locating block, a groove side face and a groove opening face and is made of a material which can bear relatively high temperature. The slide glass cover plate forms a capillary crack when combined with a piece of slide glass, the capillary crack can take a capillary siphon effect to enable a sampling reagent to uniformly cover a sample on the slide glass by virtue of the capillary siphon effect, and the sampling reagent can also be directly inserted into a reagent solution to be uniformly heated and insulated. The steps of transferring the sample into a cooking device, which can be heated, and then taking out the sample are not needed, and fully-automatic sample treatment can be realized when the slide glass sample is always in the same device and is combined with a reagent sampling instrument. By adoption of the cover plate, the slide glass can be freely placed either flatly or erectly according to space requirements to greatly save the space.
Description
technical field [0001] The invention belongs to the field of biological sample processing, in particular to a slide cover plate for processing biological slice specimens. Background technique [0002] The entire process of immunohistochemistry requires nearly thirty steps, including: a) dewaxing, hydration, endogenous enzyme blocking, washing, high temperature heat repair, cooling, washing, primary antibody incubation, washing, draining and wiping, Secondary antibody incubation, washing, draining and wiping, synergist incubation, washing, draining and wiping, color development, washing, staining, washing, dehydration, sealing and other steps. It is difficult to standardize the impact of differences in technical level and operating habits between hospitals. Improving the efficiency and reliability of specimen processing has become a top priority [0003] At present, during the inspection process, the reagent sample is added dropwise from the top of the slide. Before adding ...
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