Real-time cell metabolite detection device based on micro-fluidic chip

A microfluidic chip and real-time detection technology, applied in the field of cell research, can solve the problems of complex biochemical reactions and drug metabolism limitations of microfluidic chips, and achieve the effect of wide applicability and high detection sensitivity

Active Publication Date: 2016-06-08
TSINGHUA UNIV
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  • Abstract
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Problems solved by technology

In addition, the detection and analysis devices in the prior art still have many deficiencies in product enrichment, detection limi...

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  • Real-time cell metabolite detection device based on micro-fluidic chip
  • Real-time cell metabolite detection device based on micro-fluidic chip
  • Real-time cell metabolite detection device based on micro-fluidic chip

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Embodiment Construction

[0020] The present invention will be further described below in conjunction with accompanying drawing.

[0021] Such as figure 1 As shown in FIG. 3 , a microfluidic chip-based real-time detection device 100 for cell metabolites includes: a microfluidic chip 6 , an automatic sampling component, a pretreatment and enrichment component 40 and a detector 20 . A channel 7 with a cell culture chamber 7' is arranged in the microfluidic chip 6. An automatic sampling component capable of sampling from the outlet of the channel 7 is arranged around the microfluidic chip 6 . A pretreatment and enrichment component 40 is connected downstream of the automatic sampling component, which can desalt and enrich the target product of the sampled cell metabolites. Corresponding to the outlet of the pretreatment and enrichment component 40, a detector 20 is arranged to detect the target product online. In this case, the detection device of the present invention integrates the microfluidic chip ...

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Abstract

The invention provides a real-time cell metabolite detection device based on a micro-fluidic chip. The device comprises the micro-fluidic chip, an automatic sampling assembly, a pretreatment and enrichment assembly and a detector. Channels provided with cell culture rooms are arranged in the micro-fluidic chip, the automatic sampling assembly capable of conducting sampling at outlets of the channels is arranged around the micro-fluidic chip, and the pretreatment and enrichment assembly is connected to the down stream of the automatic sampling assembly and can conduct desalting and target product enriching on a cell metabolite obtained through sampling. The detector is arranged at the portion corresponding to an outlet of the pretreatment and enrichment assembly to conduct online detection on the target product. According to the detection device, the micro-fluidic chip, automatic sampling, pretreatment and enrichment and detection are integrated into a whole, and real-time online detection and analysis can be conducted on a biochemical reaction on the micro-fluidic chip and the drug metabolism process.

Description

technical field [0001] The invention relates to the technical field of cell research, in particular to a real-time detection device for cell metabolites based on a microfluidic chip. Background technique [0002] As we all know, compared with traditional cell research platforms, microfluidic chip technology has attracted widespread attention due to its low reagent consumption, easy integration, high throughput, easy operation and good reproducibility. In recent years, animal experiments have gradually been opposed by humans, and microfluidic chip technology is one of the most potential methods to replace animal experiments, and has broad application prospects. The channels in microfluidic chips are generally micron-scale channels comparable to the size of cells, which allows researchers not only to precisely manipulate cells, but also to reduce the amount of cells used and the consumption of reagents. In the prior art, using the characteristics of the microfluidic chip, the...

Claims

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Application Information

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IPC IPC(8): C12M1/34
CPCC12M1/34B01L3/5027B01L2300/0861
Inventor 林金明王俊茗李海芳
Owner TSINGHUA UNIV
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