An experimental method for 3D structure reconstruction of rat femoral vessels
An experimental method and femoral technology, applied in 3D modeling, laboratory analysis data, image analysis, etc., can solve the problems that the channel structure does not exist independently, the shape is complex and changeable, etc., the time to reach is non-hazardous, and the operation is simple , short time effect
Active Publication Date: 2018-11-13
HANGZHOU YUEBO BIOTECH CO LTD
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Studies have shown that there are a large number of channel structures inside the femur, and each channel structure does not exist independently. There are interconnected channels between the channels, which run through the entire interior of the femur, and the shape is complex and changeable.
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[0023] The technical solutions of the present invention will be further described below in conjunction with specific embodiments.
[0024] In the experiment, 3 adult rats were selected, and a total of 6 decalcified femur specimens were prepared. The experimental method of 3D structure reconstruction of rat femoral blood vessels includes the following steps:
[0025] 1). Preparation of contrast agent: First, take 40ml of MV-120BLUE reagent and 50ml of MV DIELUEN reagent and mix them evenly at a volume of 4:5 to obtain a mixed solution for use; then, add a total volume of 5 to the mixed solution 20 minutes before use. % (4.5ml) of the MV CURING AGENT reagent, mixed and stirred evenly again to obtain the contrast agent for later use;
[0026] 2). Contrast agent perfusion: Experimental rats were given general anesthesia by intraperitoneal injection of 0.05ml / 10g of chloral hydrate with a concentration of 10%. There is no muscle contraction reaction or corneal reaction when the li...
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The invention discloses a 3D structure reconstruction experimental method for blood vessels in rat femurs. The morphological structures of blood vessels in rat femurs are observed by preprocessing selected experiment rats, injecting a contrast medium, conducting decalcification to prepare a decalcified femur specimen, conducting MicroCT system overall scanning two-dimensional reconstruction and 3D structure image reconstruction, and the diameter range of blood vessels of experiment rats is further calculated to be 14.8 microns to 532.8 microns. The method has the advantages of being easy to operate, short in time, free of harm and the like, a blood vessel three-dimensional structure reconstruction graph which is vivid, visual and apparent is obtained, and observation and analysis can be more easily conducted. By means of 3D construction of blood vessels in rat femurs, more reliable diagnostic imaging foundation can be provided for patients suffering from femur, head and neck bone fractures on the aspect of operation mode selection through 3D reconstruction of blood vessels in rat femurs, the probability that femoral head necrosis or nonunion happens to patients can be further evaluated, and reliable estimation is conducted for the prognosis.
Description
field of invention [0001] The invention relates to a rat femoral blood vessel 3D structure reconstruction experiment method, which is used for observing the morphological structure of the rat femoral blood vessel. Background technique [0002] With the development of the current society and the development of aging, the number of patients with femoral problems caused by various factors is increasing. The most common femoral head necrosis is caused by alcohol, hormones, trauma, etc., which damage or block blood vessels. The blood supply of the femoral head is affected, and eventually avascular necrosis occurs. The treatment expert of Beijing Qianhai Femoral Head Hospital said: The disease can be divided into various types from different angles. It is the most common bone and joint disease. Its symptoms are poor local blood supply of the femoral head due to various reasons. Different pathogenic factors destroy the blood supply of the femoral head, which causes further ischemi...
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IPC IPC(8): G06T7/00G06T17/00
CPCG06F19/30G06T7/0012G06T17/00G06T2207/10081G06T2207/30101G16H10/40G16H50/30
Inventor 翁国忠
Owner HANGZHOU YUEBO BIOTECH CO LTD