Newcastle disease virus, avian influenza virus and avian adenovirus triple inactivated vaccine

A technology of chicken Newcastle disease virus and avian influenza virus, which is applied in the direction of vaccines, viruses, antiviral agents, etc., can solve the problems of increased susceptibility of chicken flocks to diseases, increased manpower and material burden of chicken farms, and impact on production performance, so as to achieve safety Good sex, good immunogenicity and high potency

Active Publication Date: 2016-06-15
YEBIO BIOENG OF QINGDAO
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In recent years, Newcastle disease and H9 subtype avian influenza are two important diseases that seriously threaten poultry; more and more chickens are infected with group I adenovirus type 4, and this disease is easy to cause secondary infection Epidemic diseases have brought a lot of troubles to poultry farmers, and repeated use of vaccines, especially inactivated vaccines, not only increases the burden of manpower and material resources on the chicken farm, but also affects the production performance of the chickens due to the injection stress of the chickens caught many times. increased susceptibility to disease
Coupled with the continuous mutation of strains in recent years, although a variety of vaccines have been introduced, the development of the epidemic is still uncontrollable. Therefore, it is necessary to screen for new epidemic strains to deal with the new hazards caused by mutations.

Method used

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  • Newcastle disease virus, avian influenza virus and avian adenovirus triple inactivated vaccine
  • Newcastle disease virus, avian influenza virus and avian adenovirus triple inactivated vaccine
  • Newcastle disease virus, avian influenza virus and avian adenovirus triple inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the screening of YBAV-4 strain strain

[0024] 1. Epidemiological survey Since 2010, some broiler breeders, laying hens and Ma chickens in Shandong, Jiangsu and other regions have experienced a disease characterized by high mortality and anatomical manifestations of hepatomegaly and hydropericardium. The disease was initially diagnosed as hydropericardium hepatitis syndrome caused by group I group C-4 avian adenovirus through clinical investigation and laboratory testing. In 2010, the inventor successfully isolated a virus strain from the liver of a dead chicken with typical symptoms of inclusion body hepatitis and hydropericardium in a farm in Zibo, Shandong.

[0025] 2. Virus isolation Take the liver of sick and dead chicken and grind it, add sterilized saline at a ratio of 1:5 to make a suspension; after repeated freezing and thawing 3 times, centrifuge at 3000r / min for 30min, take the supernatant; add penicillin and Streptomycin at 10,000 IU / ml each, ...

Embodiment 2

[0034] Embodiment 2: the preparation of YBAV-4 strain virus seed

[0035] (1) Study on optimal culture conditions of chicken hepatocytes

[0036] 1. The effect of cell density on cell growth. Five different densities (10,000 to 50,000 / ml, 50,000 to 100,000 / ml, 100,000 to 150,000 / ml, 150,000 to 200,000 / ml, 20 to 25 10,000 / ml) chicken liver cells were inoculated into the same batch of 25cm 2 Cell bottle, 225cm 2In cell flasks, 3000ml spinner bottles, and 10-layer cell factories, use the same batch of DMEM nutrient solution to culture under the same conditions, inoculate 5 bottles / 2 cells at each density, culture under the same conditions, and observe that the cells grow into a dense monolayer The time required and the shape of the cells.

[0037] 2, the influence of newborn bovine serum content on cell growth with the newborn bovine serum produced by the same manufacturer, add in the DMEM medium by the ratio of 6%, 8%, 10%, 12% respectively, cultivate the same batch of chicke...

Embodiment 3

[0047] Example 3: Preparation of Newcastle disease, H9 subtype avian influenza, avian adenovirus (group I, type 4) antigens

[0048] 1. The use, maintenance conditions and methods of use of the Millipore ultrafiltration concentrator should be carried out according to the instructions provided by the manufacturer.

[0049] 2. Detection of concentration effect

[0050] 2.1 Antigen titer (HA and / EID) in the virus liquid before concentration was determined by retaining sample before concentration. 50 / TCID 50 ), during the concentration process, take samples at any time to measure the antigen titer (HA) of the concentrated solution, determine the concentration multiple, retain the sample of the concentrated solution after concentration, and measure the antigen titer (HA and / EID) in the concentrated solution 50 / TCID 50 ).

[0051] 2.2 Filtrate Antigen Detection Take the filtrate when the concentration is about to end (at this time, the concentration of the antigen in the conc...

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Abstract

The invention provides a newcastle disease virus, avian influenza virus and avian adenovirus triple inactivated vaccine. TCID50 of I-group 4-type avian adenovirus YBAV-4 new strains is high in tilter, good in immunogenicity and capable of resisting attack of isolated viruses of H9 sub-type and avian adenovirus diseases in various places. The prepared vaccine is good in safety, and no local or whole-body adverse effects caused by the vaccine occur. By means of analysis of character, safety test and potency test data in a preservation state test, compared with results of single vaccines of similar products, difference of the triple vaccine is not remarkable, and the triple vaccine is stable and effective; a potency test result proves that the triple vaccine and three kinds of single-vaccine antibodies are all kept at a high level, antibody generation is faster compared with similar products, and the antibody of a control group is negative.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a triple inactivated vaccine of chicken Newcastle disease virus, avian influenza virus and avian adenovirus. Background technique [0002] Newcastle disease has a high morbidity and fatality rate, and it occurs frequently all over the world. Once it breaks out, it will bring a devastating blow to the poultry industry. It is a major infectious disease that endangers the poultry industry. OIE lists it as a Category A disease. [0003] Avian influenza is caused by type A influenza virus and is mainly prevalent in animals and humans. It is an acute and highly contagious disease with high mortality. It occurs widely in all parts of the world and often mutates. It is harmful to humans and poultry. The industry is very dangerous. [0004] According to the epidemiological investigation of group I poultry adenovirus, the disease has a high incidence in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/17A61K39/145A61K39/235A61P31/14
CPCA61K39/12A61K2039/5252A61K2039/552A61K2039/70C12N2710/10234C12N2760/16134C12N2760/18134
Inventor 宫晓李陆梅程增青刘新文胡潇
Owner YEBIO BIOENG OF QINGDAO
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