Method for constructing microorganism co-culture system for producing bacterial cellulose

Abstract

The invention discloses a method for constructing a microorganism co-culture system for producing bacterial cellulose. The method comprises the following steps: conducting seed amplification culture on acetobacter xylinum NUST4.2 and chlamydomonas in a separated mode, immobilizing chlamydomonas cells in calcium alginate by virtue of a micro-fluidic technique, and then conducting co-culture; and after culture, removing acetobacter xylinum cells and the chlamydomonas, so that pure bacterial cellulose is obtained. The chlamydomonas, as an oxygen producing microorganism adopted by the invention, can provide oxygen to the production of the bacterial cellulose in the later fermentation period, so that the problem of dissolving of oxygen caused by viscosity is solved. In addition, the acetobacter xylinum can secrete acetic acid during fermenting, and the chlamydomonas, which is a microorganism taking the acetic acid as a carbon source, can consume the acetic acid in a fermentation broth, so that ambient pH value is kept at an appropriate level. According to the scheme, a feasible solution is provided for solving the problem of dissolving of oxygen caused by excessive viscosity in the later fermentation period and for keeping the pH value stable during fermenting is provided, and a method for constructing a microorganism co-culture system for producing bacterial cellulose is provided.

Description

technical field [0001] The present invention improves the yield of bacterial cellulose by constructing a co-cultivation system of Acetobacter xylinum NUST4.2 (abbreviated as Ax.4.2) and Chlamydomonas, and realizes precise control of the cellulose micro-nano scale assembly process, specifically It involves the construction of a microbial co-culture system. Background technique [0002] Bacterial Cellulose (BC) is a new type of high-performance microbial synthetic material biosynthesized by Acetobacter xylinum. Compared with other forms of cellulose, although it has the same chemical composition, it also has special properties. Physicochemical and biological characteristics, especially the diversity of fermentation substrates and microorganisms that can be adjusted during the fermentation process. [0003] As a new biofunctional material, the production of bacterial cellulose has been industrialized. But so far, there are still many problems to be solved in the industrial pr...

AI Technical Summary

Problems solved by technology

The method uses Acetobacter xylinum to secrete acetic acid during the fermentation process, and Chlamydomonas is a microorganism with acetic acid as a carbon source, which can consume acetic acid in the fermentation broth and maintain the pH of the environment at an appropriate level. The co-cultivation of the two microorganisms can achieve Mutual benefit and symbiosis solves the problem of dissolved oxygen caused by high viscosity in the later stage of fermentation. At the same time, it can maintain a stable pH during the fermentation process and increase the yield of bacterial cellulose from 3.67g / L to 5.21g / L.

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