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Method for remedying mold contamination of tissue culture seedlings of Nakagawa-cho chrysanthemum indicum

A technology of wild chrysanthemum group and wild chrysanthemum seedlings, applied in gardening methods, botany equipment and methods, horticulture, etc., can solve the problems of material loss, polluted tissue culture seedlings, death, etc., and achieve the effect of reducing losses and eliminating pollution

Inactive Publication Date: 2016-06-22
王少荣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In sterile culture, more or less part of the seedlings are polluted for various reasons, resulting in the death of tissue cultured seedlings, resulting in the loss of some scarce materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0015] A method for rescuing the mold contamination of Naga River wild chrysanthemum tissue culture seedlings is characterized in that it includes the following technical links:

[0016] ① Disinfection in the contaminated bottle: put the Nagachuan wild chrysanthemum tissue culture contaminated bottle on the ultra-clean workbench, open the bottle cap, pour 0.1% mercury liter into the bottle until the surface height of the submerged medium is 0.3cm, and disinfect for 5 minutes;

[0017] ② Disinfection of contaminated seedlings: Use scissors to cut the middle and upper part of the Nagagawa wild chrysanthemum seedlings in the bottle and put them in 0.1% mercuric chloride for 1 min. After disinfection, rinse them with sterile water for 3-4 times;

[0018] ③ Inoculation: Use scissors to cut the sterilized Nagagawa wild chrysanthemum seedlings into stems about 0.5 cm long, and inoculate the stems into another medium bottle with tweezers, and inoculate 2 stems in each bottle;

[0019]...

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PUM

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Abstract

The invention discloses a method for remedying mold contamination of tissue culture seedlings of Nakagawa-cho chrysanthemum indicum.By utilizing the method, contamination of the tissue culture seedlings can be removed, so that the seedlings can continue growing, rare seedlings can be saved, and loss can be reduced.The method mainly includes: placing a Nakagawa-cho chrysanthemum indicum tissue culture contamination bottle on an ultra-clean bench; opening a bottle cap, pouring mercury chloride into the bottle until the surface of a culture medium is submersed, and disinfecting; cutting off the Nakagawa-cho chrysanthemum indicum tissue culture seedlings, and placing the same into mercury chloride for sterilization; cutting the tissue culture seedlings after being sterilized into stems, and connecting the stems into another culture medium bottle for re-culture.

Description

technical field [0001] The invention relates to a treatment method for the contamination of tissue cultured seedlings, more specifically, a treatment method for rescuing the mold pollution of the Naga River wild chrysanthemum tissue cultured seedlings. Background technique [0002] Plant tissue culture technology is one of the important fields of modern biotechnology. It is also the most widely used and successful field in agricultural production. It plays an important role in the preservation, reproduction, detoxification and rejuvenation of plant germplasm. Tissue culture technology is to place living organs, tissues or cells in a culture medium under sterile conditions and place them in a suitable environment for continuous culture of cells, tissues or individuals. The rapid propagation technology of plant tissue culture can not only maintain the characteristics and characteristics of fine varieties, but also accelerate the reproduction of fine varieties. In sterile cu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00
Inventor 王少荣
Owner 王少荣