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Brassica napus thermosensitive dominant nuclear sterile gene bnaatsms

A cabbage-type rapeseed, temperature-sensitive technology, applied in genetic engineering, plant genetic improvement, peptide source, etc., can solve problems such as lag

Active Publication Date: 2019-02-19
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the basic research on light- and temperature-sensitive nuclear male sterility in Brassica napus lags behind seriously, and the research on the cloning and regulation mechanism of the light- and temperature-sensitive nuclear male sterility gene in Brassica napus is still blank

Method used

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  • Brassica napus thermosensitive dominant nuclear sterile gene bnaatsms
  • Brassica napus thermosensitive dominant nuclear sterile gene bnaatsms
  • Brassica napus thermosensitive dominant nuclear sterile gene bnaatsms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Example 1: Analysis of Genetic Law of Brassica napus Thermosensitive Nuclear Dominant Sterile Material

[0034] 1. Experimental materials

[0035] The material used in this experiment is the two-type line TE5AB of Brassica napus thermosensitive nucleus dominant (12) , the sterile strain is called TE5A, and the fertile strain is called TE5B. Rapeseed napus temperature-sensitive nuclear dominant two-type line TE5AB Material source: Two sterile individual plants were obtained from the Brassica napus inbred line TE5, and the sterile individual plants were crossed with the Brassica napus inbred line TE1, and the first generation F 1 F 2 , and use TE1 as the recurrent parent to backcross to obtain a near-isogenic line.

[0036] 2. Inheritance of thermosensitive nuclear dominant sterile materials

[0037] Using classical genetic methods, the reciprocal cross F of temperature-sensitive sterile material TE5A and fertile normal Brassica napus TE1 1 , F 2 and backcross se...

Embodiment 2

[0040] 2. Example 2: Construction of a genetic map containing the BnaAtsMs segment of the temperature-sensitive dominant nuclear sterile gene BnaAtsMs in Brassica napus

[0041] 1. Experimental materials

[0042] The mapping population TE5AB of the temperature-sensitive dominant nuclear sterile gene is a near-isogenic line population constructed by continuous backcrossing with its corresponding parent TE1 (12). Sowed in Xining in the spring of 2012, and extracted DNA from 700 sterile individual plants derived from the near-isogenic line of TE5AB in the summer of 2012 (13) , for fine mapping of genes.

[0043] 2. Fine mapping of temperature-sensitive dominant nuclear sterile gene BnaAtsMs in Brassica napus

[0044] According to the results of preliminary mapping, the gene is located between the SCAR (Sequence characterized amplified region) markers SC1 and SC10 of the rape N08 linkage group. That is, the BnaAtsMs site is located between SC1 and SC10.

[0045] The nucleotid...

Embodiment 3

[0055] 3. Example 3: Isolation and functional verification of the temperature-sensitive dominant nuclear sterile gene BnaAtsMs in Brassica napus

[0056] 1. Determination of BnaAtsMs candidate genes

[0057] In this example, the Blast software in the model organisms Arabidopsis and Chinese cabbage genome websites (http: / / www.arabidopsis.org / blast, http: / / brassicadb.org / brad / index.php) was used to detect molecular markers IP004 and Between IP470, including BnaAtsMs or its homologous Bra.tsMs, sequence analysis from rapeseed or Chinese cabbage showed that there were six genes Bra018441, Bra018443, Bra018446, Bra018449, Bra018451 and Bra018457 that may be related to stamen development in this segment, And three cabbage-specific genes Bra018452, Bra018455 and Bra018456. The transcription start sites of these nine genes were determined by Blast software analysis in the Chinese cabbage genome website (http: / / brassicadb.org / brad / index.php), and the cDNAs of TE5A and TE5 were used as...

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Abstract

The invention discloses a cabbage type rape temperature sensitive type dominant genic male sterility gene BnaAtsMs, and relates to the technical field of oilseed rape breeding. The nucleotide sequence of the cabbage type rape temperature sensitive type dominant genic male sterility gene BnaAtsMs is shown in SEQ ID NO:1, and the coded amino acid sequence of the cabbage type rape temperature sensitive type dominant genic male sterility gene BnaAtsMs is shown in SEQ ID NO:2. The cabbage type rape temperature sensitive type dominant genic male sterility gene BnaAtsMs can be used for breeding cabbage type rape temperature sensitive type dominant male sterile lines, and can also be used for molecular marker-assisted breeding of cabbage type rape temperature sensitive type dominant genic male sterile lines, maintainer lines and restoring lines. The BnaAtsMs gene sequence is utilized for being combined with the genetic engineering technology for developing an oilseed rape intelligent sterile system; BnaAtsMs gene sequence information is utilized for achieving marker-assisted selection breeding, and oilseed rape and related crop two-line hybrid seed production can be achieved.

Description

technical field [0001] The invention relates to the technical field of rapeseed breeding, in particular to a temperature-sensitive dominant nuclear sterile gene BnaAtsMs in Brassica napus; in particular, it relates to the isolation and functional verification of a temperature-sensitive dominant nuclear male sterility gene BnaAtsMs in rapeseed And the application can speed up the selection and breeding of the two-line method of nuclear male sterility in rapeseed and other crops, so as to obtain 100% sterile populations during seed reproduction. Background technique [0002] Male sterility can be divided into cytoplasmic male sterility (CMS) and nuclear male sterility (GMS). Cytoplasmic male sterility has been known for more than 100 years and is widely used to produce hybrids in crops such as rice, corn and canola. Utilization of heterosis in rapeseed is one of the effective ways to solve the current problems faced by the rapeseed industry. The key issue in the utilization ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12Q1/6895
CPCC07K14/415C12Q1/6895C12Q2600/13
Inventor 曾新华吴刚闫晓红武玉花朱莉李均刘芳罗军玲
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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