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Method for simultaneously determining content of 6 types of water-soluble vitamins by HPLC-MS/MS (High Performance Liquid Chromatography-Mass Spectrometry/Mass Spectrometry)

A technology for water-soluble vitamins and vitamins, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problem of few types of vitamins measured at the same time, and achieve the effect of being beneficial to identification and measurement, convenient operation and fast speed.

Active Publication Date: 2016-06-22
NEW HOPE LIUHE +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiency that there are few types of vitamins simultaneously in the prior art of using HPLC-MS / MS to measure the content of vitamins, and provide a method for simultaneously measuring the contents of six water-soluble vitamins by HPLC-MS / MS

Method used

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  • Method for simultaneously determining content of 6 types of water-soluble vitamins by HPLC-MS/MS (High Performance Liquid Chromatography-Mass Spectrometry/Mass Spectrometry)
  • Method for simultaneously determining content of 6 types of water-soluble vitamins by HPLC-MS/MS (High Performance Liquid Chromatography-Mass Spectrometry/Mass Spectrometry)
  • Method for simultaneously determining content of 6 types of water-soluble vitamins by HPLC-MS/MS (High Performance Liquid Chromatography-Mass Spectrometry/Mass Spectrometry)

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Experimental program
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Effect test

Embodiment 1

[0055] 1. Pretreatment:

[0056] Pretreatment of the material to be tested: Weigh 2.0g of laying hen feed with an electronic balance, dissolve the laying hen feed with 20ml of ammonium acetate solution with a concentration of 0.02mol / L and a pH of 4, extract it ultrasonically for 20min, cool to a volume of 100mL, and centrifuge , and filter with a 0.22 μm aqueous phase filter membrane to obtain the sample to be tested;

[0057] Standard sample pretreatment: a. Accurately weigh 2.0g of thiamine, riboflavin, niacin, pyridoxine, biotin, and cobalamin standard, and prepare them with 0.02mol ammonium acetate (pH=4.0) Standard solution with a mass concentration of 1000.0mg / L;

[0058] b. Accurately pipette an appropriate amount of standard solution for mixing, and configure it as a standard working intermediate solution. VB in the standard working intermediate solution 1 , VB 3 The mass concentration is 1mg / L, VB 2 , VB 7 , VB 12 The mass concentration is 10mg / L, VB 6 The mas...

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Abstract

The invention discloses a method for simultaneously determining the content of 6 types of water-soluble vitamins by HPLC-MS / MS (High Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry). According to the method disclosed by the invention, the vitamins in feed are extracted by adopting an ammonium acetate solution with the concentration of 0.02mol / L and the pH of 4, and are detected by adopting of a chromatographic column of Capcell PAKADME (2.1mmI.D*150mm, 3 microns); and the content of the 6 types of vitamins including thiamine, riboflavin, nicotinic acid, pyridoxine, biotin and cobalamin in a material to be detected can be simultaneously determined. The method is high in determination precision, high in speed and convenient to operate.

Description

technical field [0001] The invention relates to a method for determining the content of vitamins, in particular to a method for simultaneously determining the contents of six water-soluble vitamins by HPLC-MS / MS. Background technique [0002] Vitamin is an organic compound necessary to maintain life activities and plays an important role in growth, metabolism and development. With the continuous development of animal husbandry and the gradual scale of livestock and poultry breeding, in order to meet the growing consumption demand of livestock and poultry, the nutrition and health of livestock and poultry are getting more and more attention. As an important substance to maintain the health of animals, vitamins are indispensable in livestock and poultry feed. The amount of various vitamins in the feed must meet the requirements of the animal body to ensure the healthy growth of the animal and produce products. If it is missing or insufficient, it will cause a special deficien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/027G01N2030/884
Inventor 朱正鹏梁玉树何开蓉易锡斌汪春霞陈霞王虎黄李蓉
Owner NEW HOPE LIUHE
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