A kind of culture medium and cultivation method of liquid strain of Grifola frondosa

A technology of liquid strains and cultivation methods, applied in liquid fertilizers, botany equipment and methods, cultivation, etc., can solve the problems of susceptibility to yellow rot virus, affecting the yield of Grifola frondosa, susceptibility to viruses, etc., to shorten the age of bacteria and growth cycle, shorten the strain cycle, shorten the effect of germination time

Active Publication Date: 2019-11-05
东莞市华蕈生物科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

[0006] However, the liquid strain of Grifola frondosa that is cultivated by the above-mentioned liquid medium is easily infected with viruses during the cultivation process, especially easily infected with yellow rot virus, which causes large-scale rot and yellowing of Grifola frondosa, which seriously affects the quality of Grifola frondosa. output

Method used

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  • A kind of culture medium and cultivation method of liquid strain of Grifola frondosa

Examples

Experimental program
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Effect test

Embodiment 1

[0038] The nutrient solution of described Grifola frondosa liquid strain comprises following components and parts by weight thereof:

[0039] 70 parts of glucose, 8 parts of soybean flour, 2 parts of wheat bran, 2 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, 0.05 parts of defoaming agent, 4 parts of growing agent and 600 parts of water; Sodium acid diester is composed of 4:3 by weight.

[0040] The cultivation method of described Grifola frondosa liquid strain:

[0041] Cultivation of strains in S1 slant test tubes: put the above-mentioned Grifola frondosa liquid strain culture solution in a test tube, then place the test tube at 123°C for 40 minutes to sterilize, after cooling, dry it in an incubator at 36°C, and put it in 2 days later. The pure strain of Grifola frondosa was inoculated on the solid medium in the inclined test tube, and cultivated for 10 days at a constant temperature of 22°C to obtain the inclined test tube strain;

[0042] Cultiv...

Embodiment 2

[0047] The nutrient solution of described Grifola frondosa liquid strain comprises following components and parts by weight thereof:

[0048] 76 parts of glucose, 12 parts of soybean flour, 4 parts of wheat bran, 3 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, 0.06 parts of defoamer, 6 parts of growth promoter and 640 parts of water; Sodium acid diester is composed of 5:2 by weight.

[0049] The breeding method of the Grifola frondosa liquid strain is similar to Example 1.

Embodiment 3

[0051] The nutrient solution of described Grifola frondosa liquid strain comprises following components and parts by weight thereof:

[0052] 80 parts of glucose, 16 parts of soybean flour, 6 parts of wheat bran, 4 parts of potassium dihydrogen phosphate, 5 parts of magnesium sulfate, 0.1 part of defoamer, 8 parts of growth promoter and 700 parts of water; Sodium acid diester is composed of 6:1 by weight.

[0053] The breeding method of the Grifola frondosa liquid strain is similar to Example 1.

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Abstract

The invention belongs to the technical field of edible fungi cultivation and particularly relates to a culture solution and a culture method for grifola frondosa liquid strains. The culture solution for grifola frondosa liquid strains provided by the invention mainly is prepared from glucose, soybean meal, wheat bran, monopotassium phosphate, magnesium sulfate, a defoamer, a growth substance and water. The culture solution for grifola frondosa liquid strains provided by the invention is capable of quickening the growth speed of culture, improving the vigor and vitality of the strains, and shortening the fungus age and growth cycle of grifola frondosa; furthermore, the liquid strains cultured by the culture solution are healthy and strong in development, high in anti-bacterial and anti-virus capability, uniform in fungus age and uniform in growth, and is capable of comprehensively improving the quality and yield of the grifola frondosa liquid strains and lowering the production cost.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a culture solution and a cultivation method of liquid strains of Grifola frondosa. Background technique [0002] Grifda frondosa (Grifda frondosa) belongs to the Basidiomycotina subphylum Phylomycetes, and the genus Polyporus of the order Aphyllomycetes. The fruiting body of Grifola frondosa is rich in essential amino acids and vitamins, and has high nutritional value, and its meat is soft, crisp and delicious when young, and is deeply loved by people. The fruiting body of Grifola frondosa also contains β-glucan, which has the functions of preventing diabetes, anti-tumor and anti-AIDS virus, and has high medicinal value. [0003] Because the quantity of wild Grifola frondosa in nature is extremely small, and presents the trend of decline year by year, its quantity can not satisfy the demand of market far away. At present, the artificial cultivation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/20A01G18/40
CPCA01G18/00C05B7/00C05G3/00C05G5/23C05D5/00C05F5/002C05F11/00
Inventor 郑列宜
Owner 东莞市华蕈生物科技有限公司
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