Porcine circovirus type 2 antibody rapid detection chromatography test strip and preparation method
A porcine circovirus and type 2 antibody technology, applied in the field of veterinary biology, can solve the problems of inconvenient detection of a large number of samples and high operational requirements, and achieve good hydrophilicity and immunogenicity, fast and effective detection, and convenient production.
Active Publication Date: 2017-09-19
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
View PDF4 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
IIF and IPMA are more classic, but have high requirements for operation and are not convenient for the detection of a large number of samples. ELISA is simple and reliable in operation and has good specificity, but requires expensive instruments
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0050] The present invention will be further described below in conjunction with the accompanying drawings. It should be noted that this embodiment is based on the technical solution, and provides detailed implementation and specific operation process, but the protection scope of the present invention is not limited to the present invention. Example.
[0051] Such as figure 1 As shown, the porcine circovirus type 2 antibody rapid detection chromatographic test strip includes a support layer 1, the support layer 1, a sample application layer 2, a detection layer 4 and an absorption layer 5; it also includes a gold-labeled protein release pad 3. The gold-labeled protein release pad 3 includes a glass fiber membrane and colloidal gold-labeled recombinant protein G embedded in the glass fiber membrane; the detection layer 4 is provided with a detection zone 6 and a quality control zone 7, the detection zone The dominant antigenic epitope peptide on the porcine circovirus type 2 n...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention relates to a rapid-detection chromatographic test strip for establishment of epitope peptides of dominant antigens in porcine circovirus and a preparation method, which can be used for rapidly detecting antibodies in blood or serum of a pig. The test strip comprises a supporting layer, a sampling layer, a gold-labeled protein release pad, a detection layer and an absorbing layer, wherein the sampling layer, the gold-labeled protein release pad, the detection layer and the absorbing layer are arranged on the supporting layer in sequence; colloidal-gold-labeled recombinant G protein is buried in the gold-labeled protein release pad; a detection zone and a quality control zone are arranged on the detection layer; the epitope peptides of the dominant antigens on nucleocapsid protein of porcine circovirus-2 are fixed in the detection zone; IgG of purified ProteinG immune rabbit serum is fixed in the quality control zone. The test strip is simple in operation, good in repeatability and high in sensitivity, the result is fast and intuitive, the process is simple, so that the test strip can be prepared by large batch; the test strip is low in cost, is suitable for large-batch site detection at the base level and can be used for early rapid detection of PCV2 infection of pig groups.
Description
technical field [0001] The invention relates to the field of veterinary biological technology, in particular to a chromatography test strip for rapid detection of porcine circovirus type 2 antibody and a preparation method thereof, which is mainly used for antibody monitoring of porcine circovirus type 2. Background technique [0002] Porcine circovirus (PCV) has two serotypes: PCV1 and PCV2, among which PCV1 is less pathogenic to pigs and widely exists in normal pig herds and pig-derived cells; while PCV2 is extremely harmful to pigs, Can cause a variety of disease syndromes, such as post-weaning multisystem failure syndrome, dermatitis and nephrotic syndrome, and reproductive disorders. In addition, the disease can cause multi-system, multi-stage, and all-round damage to the pig industry, causing direct or indirect losses. In order to better control porcine circovirus, it is necessary to understand and grasp the infection situation of PCV2 (porcine circovirus type 2) in p...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/569G01N33/558
CPCG01N33/558G01N33/56983G01N33/6854G01N2469/20
Inventor 孙世琪张韵郭慧琛智晓莹魏衍全常艳燕郜原
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI