SNP chemical regulation method for increasing thermo-sensitive genic male sterile wheat BNS maturing rate

Abstract

The invention discloses an SNP chemical regulation method for increasing the thermo-sensitive genic male sterile wheat BNS maturing rate. The method comprises the steps that thermo-sensitive genic male sterile wheat BNS is sown on the fertile period sowing condition to form a fertile line, beginning from stamen development to the stamen and pistil primordium differentiation period till ears of wheat are just completely extracted from flag leaf sheaths, a sodium nitroprusside solution with the molar concentration being 1000 micromol/L is adopted for performing complete-plant spraying treatment on BNS plants every four days from the first day, the spraying amount of 50-80 L/mu, and other steps are executed referring to a common field production management mode. According to field production, after the sodium nitroprusside solution with the molar concentration being 1000 micromol/L is adopted for treatment, compared with natural growth control and clean water spraying control, the maturing rates obtained through statistics of the domestic law are improved by 26.61% and 29.58% respectively, and the maturing rates obtained through statistics of the international law are improved by 45.85% and 40.66% respectively. The method is relatively easy to implement, low in input cost and capable of saving time and labor and has the wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of chemical regulation and control of BNS seed setting rate of thermosensitive genic male sterile wheat, and in particular relates to a SNP chemical regulation method for improving the BNS seed setting rate of thermosensitive genic male sterile wheat. Background technique [0002] The utilization of wheat photothermo-sensitive male sterility (PTSMS) is a major advance in the research of hybrid wheat. The wheat light-temperature-sensitive sterile lines discovered in my country, such as ES, C49S, ZP, BS, YS and BNS, have become important test materials for hybrid wheat research. [0003] Thermosensitive genic male sterile wheat BNS was successfully bred by Ru Zhengang in 2002. BNS has the characteristics of complete sterility and complete fertility transformation, and the trend of fertility change is: complete abortion-semi-sterility-complete fertility. Low temperature is the main reason for affecting the fe...

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Problems solved by technology

In theory, normal autumn sowing cultivation can also be adopted, but after the stamens have developed to the stage of differentiation of the pistil primordium, they can be transferred to the greenhouse for cultivation, and plastic sheds are used to keep them warm until mature trinuclear pollen grains are formed, especially in the pistil primordia. From the basal differentiation period to the tetrad formation period, ensure that the minimum temperature is higher than 12°C, which is expensive in large-scale seed production and difficult to popularize and apply

Under the condition of late sowing in northern Henan, there are few studies on the use of exogenous substances to increase the seed setting rate of BNS under the condition of fertile sowing date. So far, the use of sodium nitroprusside (SNP) spraying treatment to improve the BNS of thermosensitive genic male sterile wheat The research on seed setting rate under the condition of fertile sowing date has not been reported yet

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