Directly competitive ELISA detection kit for O type FMDV antibody

A detection kit, a direct technology, applied in the biological field, can solve the problems of long test cycle, detection speed limit, etc., and achieve the effects of shortened detection time, good competitive activity, and good biological safety

Active Publication Date: 2016-07-27
ZHENGZHOU ZHONGDAO BIOTECHNOLOGY CO LTD +1
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AI Technical Summary

Problems solved by technology

VN test is the most classic serological diagnosis method, but this method requires the use of cells for virus culture, and the test period is long
Compared with the VN test, both liquid-phase blocking ELISA and solid-phase blocking ELISA shorten the test time, but still require multiple antibody incubations and washings, and the detection speed is limited

Method used

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  • Directly competitive ELISA detection kit for O type FMDV antibody
  • Directly competitive ELISA detection kit for O type FMDV antibody
  • Directly competitive ELISA detection kit for O type FMDV antibody

Examples

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Embodiment Construction

[0018] The O-type FMDV antibody direct competition ELISA detection kit according to the present invention contains an enzyme label plate, a washing solution, a sample diluent, an HRP-labeled rabbit antibody, a substrate A solution, a substrate B solution, a stop solution, a positive control serum, a negative Control serum and plate sealing film, wherein the enzyme plate is coated with O-type FMDV genetic engineering antigen.

[0019] The source (or preparation process) of each reagent in the kit of the present invention, the testing principle and testing process of the kit will be introduced in detail below.

[0020] The main sources of raw materials in the following examples are: O-type FMDV, A-type FMDV and Asian I-type FMDV antibody liquid-phase blocking ELISA kits were purchased from Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences. Other reagents and materials were obtained from commercial sources. The experimental methods described are con...

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Abstract

Provided is a directly competitive ELISA detection kit for an O type FMDV antibody.An elisa plate, washing liquid, sample diluent, HRP marked rabbit anti, substrate A liquid, substrate B liquid, stop buffer, positive control serum, negative control serum and a microplate sealer are arranged in the detection kit.The elisa plate is coated with an O type FMDV gene engineering antigen.According to the detection kit, a one-step method is adopted for sample feeding, and therefore the detection time is shortened.The HRP marked rabbit anti is used for replacing bethyl, animal species variations are eliminated, and serum samples of pigs, cattle, sheep and the like can be detected.The kit can overcome the defects of an existing serological diagnosis technology, has the advantages of being sensitive, specific, fast, easy to prepare and the like, and can be widely applied to O type FMD vaccine immunity effect monitoring and epidemiological investigation.

Description

technical field [0001] The invention relates to a direct competition ELISA kit for detecting O-type FMDV antibodies, belonging to the field of biotechnology. Background technique [0002] Foot-and-mouth disease (FMD) is a highly contagious disease, and pigs, cattle, sheep and other cloven-hoofed animals are most susceptible. Foot-and-mouth disease virus (foot-and-mouth disease virus, FMDV) currently has seven serotypes, named O, A, C, South Africa I (SAT1), South Africa II (SAT2), South Africa III (SAT3) and Asia I (Asia1) , There is no cross immune protection between different FMDV serotypes. From 2005 to 2014, my country reported a total of 115 FMD outbreaks to the World Organization for Animal Health (OIE), including 46 Asia1-type outbreaks, 38 O-type outbreaks, and 31 A-type outbreaks. After 2009, the O-type and A-type FMD outbreaks were mainly prevalent in my country, among which the O-type FMDV had strong variability and spread widely, seriously endangering the devel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N33/56983
Inventor 张杰王盼曾小宇苗银萍卜攀攀柴素真李伟豪鲁龙任宝红余清卫赵林萍
Owner ZHENGZHOU ZHONGDAO BIOTECHNOLOGY CO LTD
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