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Lung qi-blood barrier injury model as well as establishment method and application thereof

A technology of air-blood barrier and injury model, which is applied in the field of medical models, can solve problems such as inappropriateness, damage to the integrity of alveolar epithelial cells and pulmonary microvascular endothelial cells, and the inability to truly and comprehensively reflect the state of the lung air-blood barrier. Accurately evaluate the effectiveness of the method

Pending Publication Date: 2022-05-31
闻庆平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies and previous literature reports have shown that the integrity of alveolar epithelial cells and pulmonary microvascular endothelial cells is severely damaged when acute pancreatitis lung injury occurs. Therefore, the existing technology mostly adopts 2D or 3D cell culture methods. Changes in cells and / or pulmonary microvascular endothelial cells to evaluate lung injury repair drugs for acute pancreatitis, but because cell experiments cannot reproduce the changes in the matrix structure of the lung air-blood barrier during injury and repair, it cannot truly and comprehensively reflect the lung air-blood barrier In the process of injury and repair, it is not suitable as an experimental model to explore the specific mechanism of lung air-blood barrier injury or repair or to study the effect of drugs on the lung air-blood barrier

Method used

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  • Lung qi-blood barrier injury model as well as establishment method and application thereof
  • Lung qi-blood barrier injury model as well as establishment method and application thereof
  • Lung qi-blood barrier injury model as well as establishment method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] Precise lung slice 4 (PCLS) production, such as figure 1 As shown, specifically:

[0106] (1) After the SD rats were anesthetized, the inferior vena cava was cut open to drain the blood from the lungs, the trachea was exposed, and a small incision was made on the front side of the cartilage belt, so that the mouse gavage needle could pass through. A 1ml syringe draws 37°C agarose and slowly injects it into the lungs. After solidification, the lungs inflated by the agarose are excised and stored in cold PBS.

[0107] (2) Fix the lung and trachea side of the agarose inflated mouse on the plunger of the metal syringe with glue, and trim the excess tissue beyond the edge of the plunger; move the plunger downward so that the sides of the metal syringe are upward Move to form a well with the lung glued to the bottom, no more than a few centimeters deep. Pour the agarose at 37°C into the well, cool and solidify, put the syringe into the automatic slicer, and slice the lung t...

Embodiment 2

[0110] Establishment of lung air-blood barrier injury model, such as Figure 2-5 As shown, specifically:

[0111] (1) The culture mold includes 4 culture units that can be used alone or at the same time, and each culture unit includes a culture chamber, a limiting part, a micro-flow pump and an air pump.

[0112] (2) The cultivation chamber body 1 is cylindrical, the bottom is a plane, and the inner diameter of the bottom is about 40mm. There are liquid inflow channels 12 for input of medium, liquid outflow channels 13 for medium output, gas inflow channels 14 for gas inflow and gas outflow channels 15 for gas output, liquid inflow channels 12 and liquid outflow channels The channel 13 is arranged symmetrically with respect to the axis line of the culture chamber, the gas inflow channel 14 and the gas outflow channel 15 are arranged symmetrically with respect to the axis line of the culture room, the liquid inflow channel 12 is connected with the micro-flow pump, and the liqu...

Embodiment 3

[0118] Bone marrow mesenchymal stem cell–derived dexosomes (BMSC-Exos) were used to repair the lung air-blood barrier damage model:

[0119] (1) Rat BMSCs were isolated, cultured, proliferated and identified in vitro.

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Abstract

The invention provides a lung qi-blood barrier injury model as well as an establishment method and application thereof. In order to overcome the defects that an existing cell experiment cannot reproduce a lung qi-blood barrier matrix structure, cannot reflect the damage or repair process and mechanism of a lung qi-blood barrier on a tissue level, a protein level, a gene level and an ultrastructure, cannot reproduce the damage or repair process of the lung qi-blood barrier, and the like. The invention provides a lung qi and blood barrier injury model which is established by culturing a precise lung slice in a culture mold of a lipopolysaccharide-containing culture medium. The model not only can dynamically observe the lung qi-blood barrier injury and repair process in real time, but also can be used for exploring the drug repair mechanism after the lung qi-blood barrier injury, is used for developing or screening drugs for repairing the lung qi-blood barrier injury, and can provide a more accurate evaluation method for the drugs for repairing the lung qi-blood barrier injury.

Description

technical field [0001] The invention belongs to the technical field of medical models, and in particular relates to a lung-qi-blood barrier damage model, its establishment method and its application. Background technique [0002] Acute pancreatitis-associated lung injury (APALI) is the most common and serious early complication of severe acute pancreatitis. Its pathogenesis is complicated and its treatment is difficult. There is no effective treatment at present. One of the most important reasons for the occurrence and development of APALI is that the uncontrolled systemic inflammatory response induces the destruction of the lung-air-blood barrier, and how to rebuild the lung-air-blood barrier is an urgent problem to be solved. [0003] The structure and function of alveolar epithelial cells (AEC) and pulmonary microvascular endothelial cells (PMVEC) are important components of the lung air-blood barrier. Previous studies and previous literature reports have shown that the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12Q1/02C12M3/00C12M1/04C12M1/00
CPCC12N5/0688G01N33/5088C12M21/08C12M23/38C12M23/24C12M23/22C12M25/04C12M29/00C12N2501/052C12N2503/02G01N2500/10
Inventor 闻庆平武平周国华武小琪王茵
Owner 闻庆平
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