Unicellular microorganism fermentation system and method

Abstract

The invention relates to a unicellular microorganism fermentation system and method and belongs to organism fermentation equipment and methods. The unicellular microorganism fermentation system and method solve the problem that the pH value is not easy to control in the fermentation process in an existing device and the problems that due to too many culture expanding times of seeds in each round of fermentation, too long time is consumed, and cost is too high in an existing fermentation device. The fermentation system comprises a fermentation tank device and N levels of seed tank devices. The fermentation tank device comprises a culture tank, a neutralizer storage jar, a circulating pump and a programmable logic controller. The constitution and structure of all the levels of seed tank devices are identical with those of the fermentation tank device, and only the dimension of the all the levels of seed tank devices is different from that of the fermentation tank device. The fermentation method comprises the steps of pre-loading, seed culture expanding, temporary seed storage, fermentation, and the Nth level seed re-culture and circulation. By means of the unicellular microorganism fermentation system and method, the seed culture expanding time is shortened, contamination risks are lowered and the time and cost for fermentation production are greatly saved; besides, the adding precision of a neutralizer is improved, a pipeline is prevented from scaling and then being blocked, the pH value is easier to control, and the level of fermentation production is increased.

Description

technical field [0001] The invention belongs to biological fermentation equipment and methods, in particular to a single-cell microbial fermentation system and a fermentation method thereof. Background technique [0002] Many important products, including various feedstuffs, food additives and APIs, are mainly obtained through fermentation. Among them, many single-cell microorganisms, such as bacteria and yeast, are used as production bacteria, and generally do not need to be subcultured by spores. The fermentation cycle is relatively short, and the frequency of tank placement is relatively high. High-frequency cultivation of seeds is required, and the workload is relatively large; On the one hand, with the development of fermentation technology, the volume of fermentation tanks has gradually expanded to 200m 3 ~500m 3 , The number of stages of seed tanks is also gradually increasing, starting from shake flasks, and finally to large fermenters, and seed expansion requires ...

AI Technical Summary

Problems solved by technology

[0003] The invention provides a single-cell microbial fermentation system and a fermentation method thereof, which solves the problem that the pH value is not easy to control in the fermentation process of the existing device, and solves the problem of too many times of seed expansion in each round of fermentation in the existing fermentation method, which leads to time-consuming The problem of too long and high cost; to reduce the number of seed culture stages and cycles, optimize the addition of neutralizers, reduce the cost of seed expansion, and improve fermentation production efficiency

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